Tanreqing Injection Attenuates Lipopolysaccharide-Induced Airway Inflammation through MAPKNF-κB Signaling Pathways in Rats Model
Joint Authors
Mao, Bing
Liu, Wei
Jiang, Hong-li
Cai, Lin-li
Yan, Min
Dong, Shou-jin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-05
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Background.
Tanreqing injection (TRQ) is a commonly used herbal patent medicine for treating inflammatory airway diseases in view of its outstanding anti-inflammatory properties.
In this study, we explored the signaling pathways involved in contributions of TRQ to LPS-induced airway inflammation in rats.
Methods/Design.
Adult male Sprague Dawley (SD) rats randomly divided into different groups received intratracheal instillation of LPS and/or intraperitoneal injection of TRQ.
Bronchoalveolar Lavage Fluid (BALF) and lung samples were collected at 24 h, 48 h, and 96 h after TRQ administration.
Protein and mRNA levels of tumor necrosis factor- (TNF-) α, Interleukin- (IL-) 1β, IL-6, and IL-8 in BALF and lung homogenate were observed by ELISA and real-time PCR, respectively.
Lung sections were stained for p38 MAPK and NF-κB detection by immunohistochemistry.
Phospho-p38 MAPK, phosphor-extracellular signal-regulated kinases ERK1/2, phospho-SAPK/JNK, phospho-NF-κB p65, phospho-IKKα/β, and phospho-IκB-α were measured by western blot analysis.
Results.
The results showed that TRQ significantly counteracted LPS-stimulated release of TNF-α, IL-1β, IL-6, and IL-8, attenuated cells influx in BALF, mitigated mucus hypersecretion, suppressed phosphorylation of NF-κB p65, IκB-α, ΙKKα/β, ERK1/2, JNK, and p38 MAPK, and inhibited p38 MAPK and NF-κB p65 expression in rat lungs.
Conclusions.
Results of the current research indicate that TRQ possesses potent exhibitory effects in LPS-induced airway inflammation by, at least partially, suppressing the MAPKs and NF-κB signaling pathways, in a general dose-dependent manner.
American Psychological Association (APA)
Liu, Wei& Jiang, Hong-li& Cai, Lin-li& Yan, Min& Dong, Shou-jin& Mao, Bing. 2016. Tanreqing Injection Attenuates Lipopolysaccharide-Induced Airway Inflammation through MAPKNF-κB Signaling Pathways in Rats Model. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1104201
Modern Language Association (MLA)
Liu, Wei…[et al.]. Tanreqing Injection Attenuates Lipopolysaccharide-Induced Airway Inflammation through MAPKNF-κB Signaling Pathways in Rats Model. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-15.
https://search.emarefa.net/detail/BIM-1104201
American Medical Association (AMA)
Liu, Wei& Jiang, Hong-li& Cai, Lin-li& Yan, Min& Dong, Shou-jin& Mao, Bing. Tanreqing Injection Attenuates Lipopolysaccharide-Induced Airway Inflammation through MAPKNF-κB Signaling Pathways in Rats Model. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1104201
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104201