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Berberine Protects Human Umbilical Vein Endothelial Cells against LPS-Induced Apoptosis by Blocking JNK-Mediated Signaling
المؤلفون المشاركون
Zhang, Dayong
Pan, Jianping
Guo, Junping
Wang, Lijun
Wang, Linyao
Qian, Senmi
Fang, Jie
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-07-13
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Endothelial dysfunction is a critical factor during the initiation of atherosclerosis.
Berberine has a beneficial effect on endothelial function; however, the underlying mechanisms remain unclear.
In this study, we investigated the effects of berberine on lipopolysaccharide- (LPS-) induced apoptosis in human umbilical vein endothelial cells (HUVECs) and the molecular mechanisms mediating the effect.
The effects of berberine on LPS-induced cell apoptosis and viability were measured with 5-ethynyl-2′-deoxyuridine staining, flow cytometry, and Cell Counting Kit-8 assays.
The expression and/or activation of proapoptotic and antiapoptotic proteins or signaling pathways, including caspase-3, poly(ADP-ribose) polymerase, myeloid cell leukemia-1 (MCL-1), p38 mitogen-activated protein kinase, C-Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase, were determined with western blotting.
The malondialdehyde levels, superoxide dismutase (SOD) activity, and production of proinflammatory cytokines were measured with enzyme-linked immunosorbent assays.
The results demonstrated that berberine pretreatment protected HUVECs from LPS-induced apoptosis, attenuated LPS-induced injury, inhibited LPS-induced JNK phosphorylation, increased MCL-1 expression and SOD activity, and decreased proinflammatory cytokine production.
The effects of berberine on LPS-treated HUVECs were prevented by SP600125, a JNK-specific inhibitor.
Thus, berberine might be a potential candidate in the treatment of endothelial cell injury-related vascular diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Guo, Junping& Wang, Lijun& Wang, Linyao& Qian, Senmi& Zhang, Dayong& Fang, Jie…[et al.]. 2016. Berberine Protects Human Umbilical Vein Endothelial Cells against LPS-Induced Apoptosis by Blocking JNK-Mediated Signaling. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1104313
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Guo, Junping…[et al.]. Berberine Protects Human Umbilical Vein Endothelial Cells against LPS-Induced Apoptosis by Blocking JNK-Mediated Signaling. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1104313
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Guo, Junping& Wang, Lijun& Wang, Linyao& Qian, Senmi& Zhang, Dayong& Fang, Jie…[et al.]. Berberine Protects Human Umbilical Vein Endothelial Cells against LPS-Induced Apoptosis by Blocking JNK-Mediated Signaling. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1104313
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1104313
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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