Berberine Protects Human Umbilical Vein Endothelial Cells against LPS-Induced Apoptosis by Blocking JNK-Mediated Signaling
Joint Authors
Zhang, Dayong
Pan, Jianping
Guo, Junping
Wang, Lijun
Wang, Linyao
Qian, Senmi
Fang, Jie
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-13
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Endothelial dysfunction is a critical factor during the initiation of atherosclerosis.
Berberine has a beneficial effect on endothelial function; however, the underlying mechanisms remain unclear.
In this study, we investigated the effects of berberine on lipopolysaccharide- (LPS-) induced apoptosis in human umbilical vein endothelial cells (HUVECs) and the molecular mechanisms mediating the effect.
The effects of berberine on LPS-induced cell apoptosis and viability were measured with 5-ethynyl-2′-deoxyuridine staining, flow cytometry, and Cell Counting Kit-8 assays.
The expression and/or activation of proapoptotic and antiapoptotic proteins or signaling pathways, including caspase-3, poly(ADP-ribose) polymerase, myeloid cell leukemia-1 (MCL-1), p38 mitogen-activated protein kinase, C-Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase, were determined with western blotting.
The malondialdehyde levels, superoxide dismutase (SOD) activity, and production of proinflammatory cytokines were measured with enzyme-linked immunosorbent assays.
The results demonstrated that berberine pretreatment protected HUVECs from LPS-induced apoptosis, attenuated LPS-induced injury, inhibited LPS-induced JNK phosphorylation, increased MCL-1 expression and SOD activity, and decreased proinflammatory cytokine production.
The effects of berberine on LPS-treated HUVECs were prevented by SP600125, a JNK-specific inhibitor.
Thus, berberine might be a potential candidate in the treatment of endothelial cell injury-related vascular diseases.
American Psychological Association (APA)
Guo, Junping& Wang, Lijun& Wang, Linyao& Qian, Senmi& Zhang, Dayong& Fang, Jie…[et al.]. 2016. Berberine Protects Human Umbilical Vein Endothelial Cells against LPS-Induced Apoptosis by Blocking JNK-Mediated Signaling. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1104313
Modern Language Association (MLA)
Guo, Junping…[et al.]. Berberine Protects Human Umbilical Vein Endothelial Cells against LPS-Induced Apoptosis by Blocking JNK-Mediated Signaling. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1104313
American Medical Association (AMA)
Guo, Junping& Wang, Lijun& Wang, Linyao& Qian, Senmi& Zhang, Dayong& Fang, Jie…[et al.]. Berberine Protects Human Umbilical Vein Endothelial Cells against LPS-Induced Apoptosis by Blocking JNK-Mediated Signaling. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1104313
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104313