Diosgenin and 5-Methoxypsoralen Ameliorate Insulin Resistance through ER-αPI3KAkt-Signaling Pathways in HepG2 Cells
المؤلفون المشاركون
Lu, Fuer
Fang, Ke
Jiang, Shujun
Li, Fen
Wang, Dingkun
Yang, Desen
Gong, Jing
Huang, Wenya
Dong, Hui
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-08-31
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
To determine the effects and the underlying mechanism of diosgenin (DSG) and 5-methoxypsoralen (5-MOP), two main active components in the classical Chinese prescription Hu-Lu-Ba-Wan (HLBW), on insulin resistance, HepG2 cells were incubated in medium containing insulin.
Treatments with DSG, 5-MOP, and their combination were performed, respectively.
The result showed that the incubation of HepG2 cells with high concentration insulin markedly decreased glucose consumption and glycogen synthesis.
However, treatment with DSG, 5-MOP, or their combination significantly reversed the condition and increased the phosphorylated expression of estrogen receptor-α (ERα), sarcoma (Src), Akt/protein kinase B, glycogen synthase kinase-3β (GSK-3β), and the p85 regulatory subunit of phosphatidylinositol 3-kinase p85 (PI3Kp85).
At the transcriptional level, expression of the genes mentioned above also increased except for the negative regulation of GSK-3β mRNA.
The increased expression of glucose transport-4 (GLUT-4) was meanwhile observed through immunofluorescence.
Nevertheless, the synergistic effect of DSG and 5-MOP on improving glycometabolism was not obvious in the present study.
These results suggested that DSG and 5-MOP may improve insulin resistance through an ER-mediated PI3K/Akt activation pathway which may be a new strategy for type 2 diabetes mellitus, especially for women in an estrogen-deficient condition.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Fang, Ke& Dong, Hui& Jiang, Shujun& Li, Fen& Wang, Dingkun& Yang, Desen…[et al.]. 2016. Diosgenin and 5-Methoxypsoralen Ameliorate Insulin Resistance through ER-αPI3KAkt-Signaling Pathways in HepG2 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1104356
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Fang, Ke…[et al.]. Diosgenin and 5-Methoxypsoralen Ameliorate Insulin Resistance through ER-αPI3KAkt-Signaling Pathways in HepG2 Cells. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1104356
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Fang, Ke& Dong, Hui& Jiang, Shujun& Li, Fen& Wang, Dingkun& Yang, Desen…[et al.]. Diosgenin and 5-Methoxypsoralen Ameliorate Insulin Resistance through ER-αPI3KAkt-Signaling Pathways in HepG2 Cells. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1104356
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1104356
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر