Diosgenin and 5-Methoxypsoralen Ameliorate Insulin Resistance through ER-αPI3KAkt-Signaling Pathways in HepG2 Cells
Joint Authors
Lu, Fuer
Fang, Ke
Jiang, Shujun
Li, Fen
Wang, Dingkun
Yang, Desen
Gong, Jing
Huang, Wenya
Dong, Hui
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-31
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
To determine the effects and the underlying mechanism of diosgenin (DSG) and 5-methoxypsoralen (5-MOP), two main active components in the classical Chinese prescription Hu-Lu-Ba-Wan (HLBW), on insulin resistance, HepG2 cells were incubated in medium containing insulin.
Treatments with DSG, 5-MOP, and their combination were performed, respectively.
The result showed that the incubation of HepG2 cells with high concentration insulin markedly decreased glucose consumption and glycogen synthesis.
However, treatment with DSG, 5-MOP, or their combination significantly reversed the condition and increased the phosphorylated expression of estrogen receptor-α (ERα), sarcoma (Src), Akt/protein kinase B, glycogen synthase kinase-3β (GSK-3β), and the p85 regulatory subunit of phosphatidylinositol 3-kinase p85 (PI3Kp85).
At the transcriptional level, expression of the genes mentioned above also increased except for the negative regulation of GSK-3β mRNA.
The increased expression of glucose transport-4 (GLUT-4) was meanwhile observed through immunofluorescence.
Nevertheless, the synergistic effect of DSG and 5-MOP on improving glycometabolism was not obvious in the present study.
These results suggested that DSG and 5-MOP may improve insulin resistance through an ER-mediated PI3K/Akt activation pathway which may be a new strategy for type 2 diabetes mellitus, especially for women in an estrogen-deficient condition.
American Psychological Association (APA)
Fang, Ke& Dong, Hui& Jiang, Shujun& Li, Fen& Wang, Dingkun& Yang, Desen…[et al.]. 2016. Diosgenin and 5-Methoxypsoralen Ameliorate Insulin Resistance through ER-αPI3KAkt-Signaling Pathways in HepG2 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1104356
Modern Language Association (MLA)
Fang, Ke…[et al.]. Diosgenin and 5-Methoxypsoralen Ameliorate Insulin Resistance through ER-αPI3KAkt-Signaling Pathways in HepG2 Cells. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1104356
American Medical Association (AMA)
Fang, Ke& Dong, Hui& Jiang, Shujun& Li, Fen& Wang, Dingkun& Yang, Desen…[et al.]. Diosgenin and 5-Methoxypsoralen Ameliorate Insulin Resistance through ER-αPI3KAkt-Signaling Pathways in HepG2 Cells. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1104356
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104356