Reduced Representation Bisulfite Sequencing Determination of Distinctive DNA Hypermethylated Genes in the Progression to Colon Cancer in African Americans
المؤلفون المشاركون
Shokrani, Babak
Ashktorab, Hassan
Shakoori, Afnan
Zarnogi, Shatha
Sun, Xueguang
Varma, Sudhir
Lee, Edward
Laiyemo, Adeyinka O.
Washington, Kareem
Brim, Hassan
المصدر
Gastroenterology Research and Practice
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-09-01
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Background and Aims.
Many studies have focused on the determination of methylated targets in colorectal cancer.
However, few analyzed the progressive methylation in the sequence from normal to adenoma and ultimately to malignant tumors.
This is of utmost importance especially in populations such as African Americans who generally display aggressive tumors at diagnosis and for whom markers of early neoplasia are needed.
We aimed to determine methylated targets in the path to colon cancer in African American patients using Reduced Representation Bisulfite Sequencing (RRBS).
Methods.
Genomic DNA was isolated from fresh frozen tissues of patients with different colon lesions: normal, a tubular adenoma, a tubulovillous adenoma, and five cancers.
RRBS was performed on these DNA samples to identify hypermethylation.
Alignment, mapping, and confirmed CpG methylation analyses were performed.
Preferential hypermethylated pathways were determined using Ingenuity Pathway Analysis (IPA).
Results.
We identified hypermethylated CpG sites in the following genes: L3MBTL1, NKX6-2, PREX1, TRAF7, PRDM14, and NEFM with the number of CpG sites being 14, 17, 10, 16, 6, and 6, respectively, after pairwise analysis of normal versus adenoma, adenoma versus cancer, and normal versus cancer.
IPA mapped the above-mentioned hypermethylated genes to the Wnt/β-catenin, PI3k/AKT, VEGF, and JAK/STAT3 signaling pathways.
Conclusion.
This work provides insight into novel differential CpGs hypermethylation sites in colorectal carcinogenesis.
Functional analysis of the novel gene targets is needed to confirm their roles in their associated carcinogenic pathways.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ashktorab, Hassan& Shakoori, Afnan& Zarnogi, Shatha& Sun, Xueguang& Varma, Sudhir& Lee, Edward…[et al.]. 2016. Reduced Representation Bisulfite Sequencing Determination of Distinctive DNA Hypermethylated Genes in the Progression to Colon Cancer in African Americans. Gastroenterology Research and Practice،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1104611
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ashktorab, Hassan…[et al.]. Reduced Representation Bisulfite Sequencing Determination of Distinctive DNA Hypermethylated Genes in the Progression to Colon Cancer in African Americans. Gastroenterology Research and Practice Vol. 2016, no. 2016 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1104611
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ashktorab, Hassan& Shakoori, Afnan& Zarnogi, Shatha& Sun, Xueguang& Varma, Sudhir& Lee, Edward…[et al.]. Reduced Representation Bisulfite Sequencing Determination of Distinctive DNA Hypermethylated Genes in the Progression to Colon Cancer in African Americans. Gastroenterology Research and Practice. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1104611
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1104611
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر