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Reduced Representation Bisulfite Sequencing Determination of Distinctive DNA Hypermethylated Genes in the Progression to Colon Cancer in African Americans
Joint Authors
Shokrani, Babak
Ashktorab, Hassan
Shakoori, Afnan
Zarnogi, Shatha
Sun, Xueguang
Varma, Sudhir
Lee, Edward
Laiyemo, Adeyinka O.
Washington, Kareem
Brim, Hassan
Source
Gastroenterology Research and Practice
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background and Aims.
Many studies have focused on the determination of methylated targets in colorectal cancer.
However, few analyzed the progressive methylation in the sequence from normal to adenoma and ultimately to malignant tumors.
This is of utmost importance especially in populations such as African Americans who generally display aggressive tumors at diagnosis and for whom markers of early neoplasia are needed.
We aimed to determine methylated targets in the path to colon cancer in African American patients using Reduced Representation Bisulfite Sequencing (RRBS).
Methods.
Genomic DNA was isolated from fresh frozen tissues of patients with different colon lesions: normal, a tubular adenoma, a tubulovillous adenoma, and five cancers.
RRBS was performed on these DNA samples to identify hypermethylation.
Alignment, mapping, and confirmed CpG methylation analyses were performed.
Preferential hypermethylated pathways were determined using Ingenuity Pathway Analysis (IPA).
Results.
We identified hypermethylated CpG sites in the following genes: L3MBTL1, NKX6-2, PREX1, TRAF7, PRDM14, and NEFM with the number of CpG sites being 14, 17, 10, 16, 6, and 6, respectively, after pairwise analysis of normal versus adenoma, adenoma versus cancer, and normal versus cancer.
IPA mapped the above-mentioned hypermethylated genes to the Wnt/β-catenin, PI3k/AKT, VEGF, and JAK/STAT3 signaling pathways.
Conclusion.
This work provides insight into novel differential CpGs hypermethylation sites in colorectal carcinogenesis.
Functional analysis of the novel gene targets is needed to confirm their roles in their associated carcinogenic pathways.
American Psychological Association (APA)
Ashktorab, Hassan& Shakoori, Afnan& Zarnogi, Shatha& Sun, Xueguang& Varma, Sudhir& Lee, Edward…[et al.]. 2016. Reduced Representation Bisulfite Sequencing Determination of Distinctive DNA Hypermethylated Genes in the Progression to Colon Cancer in African Americans. Gastroenterology Research and Practice،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1104611
Modern Language Association (MLA)
Ashktorab, Hassan…[et al.]. Reduced Representation Bisulfite Sequencing Determination of Distinctive DNA Hypermethylated Genes in the Progression to Colon Cancer in African Americans. Gastroenterology Research and Practice Vol. 2016, no. 2016 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1104611
American Medical Association (AMA)
Ashktorab, Hassan& Shakoori, Afnan& Zarnogi, Shatha& Sun, Xueguang& Varma, Sudhir& Lee, Edward…[et al.]. Reduced Representation Bisulfite Sequencing Determination of Distinctive DNA Hypermethylated Genes in the Progression to Colon Cancer in African Americans. Gastroenterology Research and Practice. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1104611
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104611