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Differential Gene Expression during Larval Metamorphic Development in the Pearl Oyster, Pinctada fucata, Based on Transcriptome Analysis
المؤلفون المشاركون
Li, Haimei
Zhang, Bo
Huang, Guiju
Liu, Baosuo
Fan, Sigang
Zhang, Dongling
Yu, Dahui
المصدر
International Journal of Genomics
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-10-23
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
P.
fucata experiences a series of transformations in appearance, from swimming larvae to sessile juveniles, during which significant changes in gene expression likely occur.
Thus, P.
fucata could be an ideal model in which to study the molecular mechanisms of larval metamorphosis during development in invertebrates.
To study the molecular driving force behind metamorphic development in larvae of P.
fucata, transcriptomes of five larval stages (trochophore, D-shape, umbonal, eyespots, and spats) were sequenced using an Illumina HiSeq™ 2000 system and assembled and characterized with the transcripts of six tissues.
As a result, a total of 174,126 unique transcripts were assembled and 60,999 were annotated.
The number of unigenes varied among the five larval stages.
Expression profiles were distinctly different between trochophore, D-shape, umbonal, eyespots, and spats larvae.
As a result, 29 expression trends were sorted, of which eight were significant.
Among others, 80 development-related, differentially expressed unigenes (DEGs) were identified, of which the majority were homeobox-containing genes.
Most DEGs occurred among trochophore, D-shaped, and UES (umbonal, eyespots, and spats) larvae as verified by qPCR.
Principal component analysis (PCA) also revealed significant differences in expression among trochophore, D-shaped, and UES larvae with ten transcripts identified but no matching annotations.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Haimei& Zhang, Bo& Huang, Guiju& Liu, Baosuo& Fan, Sigang& Zhang, Dongling…[et al.]. 2016. Differential Gene Expression during Larval Metamorphic Development in the Pearl Oyster, Pinctada fucata, Based on Transcriptome Analysis. International Journal of Genomics،Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1106150
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Haimei…[et al.]. Differential Gene Expression during Larval Metamorphic Development in the Pearl Oyster, Pinctada fucata, Based on Transcriptome Analysis. International Journal of Genomics No. 2016 (2016), pp.1-15.
https://search.emarefa.net/detail/BIM-1106150
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Haimei& Zhang, Bo& Huang, Guiju& Liu, Baosuo& Fan, Sigang& Zhang, Dongling…[et al.]. Differential Gene Expression during Larval Metamorphic Development in the Pearl Oyster, Pinctada fucata, Based on Transcriptome Analysis. International Journal of Genomics. 2016. Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1106150
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1106150
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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