Differential Gene Expression during Larval Metamorphic Development in the Pearl Oyster, Pinctada fucata, Based on Transcriptome Analysis
Joint Authors
Li, Haimei
Zhang, Bo
Huang, Guiju
Liu, Baosuo
Fan, Sigang
Zhang, Dongling
Yu, Dahui
Source
International Journal of Genomics
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-23
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
P.
fucata experiences a series of transformations in appearance, from swimming larvae to sessile juveniles, during which significant changes in gene expression likely occur.
Thus, P.
fucata could be an ideal model in which to study the molecular mechanisms of larval metamorphosis during development in invertebrates.
To study the molecular driving force behind metamorphic development in larvae of P.
fucata, transcriptomes of five larval stages (trochophore, D-shape, umbonal, eyespots, and spats) were sequenced using an Illumina HiSeq™ 2000 system and assembled and characterized with the transcripts of six tissues.
As a result, a total of 174,126 unique transcripts were assembled and 60,999 were annotated.
The number of unigenes varied among the five larval stages.
Expression profiles were distinctly different between trochophore, D-shape, umbonal, eyespots, and spats larvae.
As a result, 29 expression trends were sorted, of which eight were significant.
Among others, 80 development-related, differentially expressed unigenes (DEGs) were identified, of which the majority were homeobox-containing genes.
Most DEGs occurred among trochophore, D-shaped, and UES (umbonal, eyespots, and spats) larvae as verified by qPCR.
Principal component analysis (PCA) also revealed significant differences in expression among trochophore, D-shaped, and UES larvae with ten transcripts identified but no matching annotations.
American Psychological Association (APA)
Li, Haimei& Zhang, Bo& Huang, Guiju& Liu, Baosuo& Fan, Sigang& Zhang, Dongling…[et al.]. 2016. Differential Gene Expression during Larval Metamorphic Development in the Pearl Oyster, Pinctada fucata, Based on Transcriptome Analysis. International Journal of Genomics،Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1106150
Modern Language Association (MLA)
Li, Haimei…[et al.]. Differential Gene Expression during Larval Metamorphic Development in the Pearl Oyster, Pinctada fucata, Based on Transcriptome Analysis. International Journal of Genomics No. 2016 (2016), pp.1-15.
https://search.emarefa.net/detail/BIM-1106150
American Medical Association (AMA)
Li, Haimei& Zhang, Bo& Huang, Guiju& Liu, Baosuo& Fan, Sigang& Zhang, Dongling…[et al.]. Differential Gene Expression during Larval Metamorphic Development in the Pearl Oyster, Pinctada fucata, Based on Transcriptome Analysis. International Journal of Genomics. 2016. Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1106150
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1106150