Novel Microdilution Method to Assess Double and Triple Antibiotic Combination Therapy In Vitro
المؤلف
المصدر
International Journal of Microbiology
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-04-18
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
An in vitro microdilution method was developed to assess double and triple combinations of antibiotics.
Five antibiotics including ciprofloxacin, amikacin, ceftazidime, piperacillin, and imipenem were tested against 10 clinical isolates of Pseudomonas aeruginosa.
Each isolate was tested against ten double and nine triple combinations of the antibiotics.
A 96-well plate was used to test three antibiotics, each one alone and in double and triple combinations against each isolate.
The minimum bacteriostatic and bactericidal concentrations in combination were determined with respect to the most potent antibiotic.
An Interaction Code (IC) was generated for each combination, where a numerical value was designated based on the 2-fold increase or decrease in the MICs with respect to the most potent antibiotic.
The results of the combinations were verified by time-kill assay at constant concentrations of the antibiotics and in a chemostat.
Only 13% of the double combinations were synergistic, whereas 5% showed antagonism.
Forty-three percent of the triple combinations were synergistic with no antagonism observed, and 100% synergism was observed in combination of ciprofloxacin, amikacin, and ceftazidime.
The presented protocol is simple and fast and can help the clinicians in the early selection of the effective antibiotic therapy for treatment of severe infections.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
El-Azizi, Mohamed. 2016. Novel Microdilution Method to Assess Double and Triple Antibiotic Combination Therapy In Vitro. International Journal of Microbiology،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1106269
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
El-Azizi, Mohamed. Novel Microdilution Method to Assess Double and Triple Antibiotic Combination Therapy In Vitro. International Journal of Microbiology No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1106269
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
El-Azizi, Mohamed. Novel Microdilution Method to Assess Double and Triple Antibiotic Combination Therapy In Vitro. International Journal of Microbiology. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1106269
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1106269
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر