Novel Microdilution Method to Assess Double and Triple Antibiotic Combination Therapy In Vitro
Author
Source
International Journal of Microbiology
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-18
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
An in vitro microdilution method was developed to assess double and triple combinations of antibiotics.
Five antibiotics including ciprofloxacin, amikacin, ceftazidime, piperacillin, and imipenem were tested against 10 clinical isolates of Pseudomonas aeruginosa.
Each isolate was tested against ten double and nine triple combinations of the antibiotics.
A 96-well plate was used to test three antibiotics, each one alone and in double and triple combinations against each isolate.
The minimum bacteriostatic and bactericidal concentrations in combination were determined with respect to the most potent antibiotic.
An Interaction Code (IC) was generated for each combination, where a numerical value was designated based on the 2-fold increase or decrease in the MICs with respect to the most potent antibiotic.
The results of the combinations were verified by time-kill assay at constant concentrations of the antibiotics and in a chemostat.
Only 13% of the double combinations were synergistic, whereas 5% showed antagonism.
Forty-three percent of the triple combinations were synergistic with no antagonism observed, and 100% synergism was observed in combination of ciprofloxacin, amikacin, and ceftazidime.
The presented protocol is simple and fast and can help the clinicians in the early selection of the effective antibiotic therapy for treatment of severe infections.
American Psychological Association (APA)
El-Azizi, Mohamed. 2016. Novel Microdilution Method to Assess Double and Triple Antibiotic Combination Therapy In Vitro. International Journal of Microbiology،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1106269
Modern Language Association (MLA)
El-Azizi, Mohamed. Novel Microdilution Method to Assess Double and Triple Antibiotic Combination Therapy In Vitro. International Journal of Microbiology No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1106269
American Medical Association (AMA)
El-Azizi, Mohamed. Novel Microdilution Method to Assess Double and Triple Antibiotic Combination Therapy In Vitro. International Journal of Microbiology. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1106269
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1106269