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Porous Lactose-Modified Chitosan Scaffold for Liver Tissue Engineering: Influence of Galactose Moieties on Cell Attachment and Mechanical Stability
المؤلفون المشاركون
Wang, Birong
Hu, Qinggang
Wan, Tao
Yang, Fengxiao
Cui, Le
Hu, Shaobo
Gong, Bo
Li, Min
Zheng, Qi Chang
المصدر
International Journal of Polymer Science
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-02-11
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Galactosylated chitosan (CTS) has been widely applied in liver tissue engineering as scaffold.
However, the influence of degree of substitution (DS) of galactose moieties on cell attachment and mechanical stability is not clear.
In this study, we synthesized the lactose-modified chitosan (Lact-CTS) with various DS of galactose moieties by Schiff base reaction and reducing action of NaBH4, characterized by FTIR.
The DS of Lact-CTS-1, Lact-CTS-2, and Lact-CTS-3 was 19.66%, 48.62%, and 66.21% through the method of potentiometric titration.
The cell attachment of hepatocytes on the CTS and Lact-CTS films was enhanced accompanied with the increase of galactose moieties on CTS chain because of the galactose ligand-receptor recognition; however, the mechanical stability of Lact-CTS-3 was reduced contributing to the extravagant hydrophilicity, which was proved using the sessile drop method.
Then, the three-dimensional Lact-CTS scaffolds were fabricated by freezing-drying technique.
The SEM images revealed the homogeneous pore bearing the favorable connectivity and the pore sizes of scaffolds with majority of 100 μm; however, the extract solution of Lact-CTS-3 scaffold significantly damaged red blood cells by hemolysis assay, indicating that exorbitant DS of Lact-CTS-3 decreased the mechanical stability and increased the toxicity.
To sum up, the Lact-CTS-2 with 48.62% of galactose moieties could facilitate the cell attachment and possess great biocompatibility and mechanical stability, indicating that Lact-CTS-2 was a promising material for liver tissue engineering.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Birong& Hu, Qinggang& Wan, Tao& Yang, Fengxiao& Cui, Le& Hu, Shaobo…[et al.]. 2016. Porous Lactose-Modified Chitosan Scaffold for Liver Tissue Engineering: Influence of Galactose Moieties on Cell Attachment and Mechanical Stability. International Journal of Polymer Science،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1106774
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Birong…[et al.]. Porous Lactose-Modified Chitosan Scaffold for Liver Tissue Engineering: Influence of Galactose Moieties on Cell Attachment and Mechanical Stability. International Journal of Polymer Science No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1106774
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Birong& Hu, Qinggang& Wan, Tao& Yang, Fengxiao& Cui, Le& Hu, Shaobo…[et al.]. Porous Lactose-Modified Chitosan Scaffold for Liver Tissue Engineering: Influence of Galactose Moieties on Cell Attachment and Mechanical Stability. International Journal of Polymer Science. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1106774
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1106774
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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