Porous Lactose-Modified Chitosan Scaffold for Liver Tissue Engineering: Influence of Galactose Moieties on Cell Attachment and Mechanical Stability
Joint Authors
Wang, Birong
Hu, Qinggang
Wan, Tao
Yang, Fengxiao
Cui, Le
Hu, Shaobo
Gong, Bo
Li, Min
Zheng, Qi Chang
Source
International Journal of Polymer Science
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Galactosylated chitosan (CTS) has been widely applied in liver tissue engineering as scaffold.
However, the influence of degree of substitution (DS) of galactose moieties on cell attachment and mechanical stability is not clear.
In this study, we synthesized the lactose-modified chitosan (Lact-CTS) with various DS of galactose moieties by Schiff base reaction and reducing action of NaBH4, characterized by FTIR.
The DS of Lact-CTS-1, Lact-CTS-2, and Lact-CTS-3 was 19.66%, 48.62%, and 66.21% through the method of potentiometric titration.
The cell attachment of hepatocytes on the CTS and Lact-CTS films was enhanced accompanied with the increase of galactose moieties on CTS chain because of the galactose ligand-receptor recognition; however, the mechanical stability of Lact-CTS-3 was reduced contributing to the extravagant hydrophilicity, which was proved using the sessile drop method.
Then, the three-dimensional Lact-CTS scaffolds were fabricated by freezing-drying technique.
The SEM images revealed the homogeneous pore bearing the favorable connectivity and the pore sizes of scaffolds with majority of 100 μm; however, the extract solution of Lact-CTS-3 scaffold significantly damaged red blood cells by hemolysis assay, indicating that exorbitant DS of Lact-CTS-3 decreased the mechanical stability and increased the toxicity.
To sum up, the Lact-CTS-2 with 48.62% of galactose moieties could facilitate the cell attachment and possess great biocompatibility and mechanical stability, indicating that Lact-CTS-2 was a promising material for liver tissue engineering.
American Psychological Association (APA)
Wang, Birong& Hu, Qinggang& Wan, Tao& Yang, Fengxiao& Cui, Le& Hu, Shaobo…[et al.]. 2016. Porous Lactose-Modified Chitosan Scaffold for Liver Tissue Engineering: Influence of Galactose Moieties on Cell Attachment and Mechanical Stability. International Journal of Polymer Science،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1106774
Modern Language Association (MLA)
Wang, Birong…[et al.]. Porous Lactose-Modified Chitosan Scaffold for Liver Tissue Engineering: Influence of Galactose Moieties on Cell Attachment and Mechanical Stability. International Journal of Polymer Science No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1106774
American Medical Association (AMA)
Wang, Birong& Hu, Qinggang& Wan, Tao& Yang, Fengxiao& Cui, Le& Hu, Shaobo…[et al.]. Porous Lactose-Modified Chitosan Scaffold for Liver Tissue Engineering: Influence of Galactose Moieties on Cell Attachment and Mechanical Stability. International Journal of Polymer Science. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1106774
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1106774