Construction of a Turn Off-On-Off Fluorescent System Based on Competitive Coordination of Cu2+ between 6,7-Dihydroxycoumarin and Pyrophosphate Ion for Sensitive Assay of Pyrophosphatase Activity
المؤلفون المشاركون
Zhang, Chengxiao
Zhao, Lingzhi
Zhao, Liu
Miao, Yanqing
Liu, Chunye
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-09-27
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The detection of pyrophosphatase (PPase) activity is of great significance in diagnosing diseases and understanding the function of PPase-related biological events.
This study constructed a turn off-on-off fluorescent system for PPase activity assay based on PPase-regulated competitive coordination of Cu2+ between a water-soluble fluorescent probe 6,7-dihydroxycoumarin (DHC) and pyrophosphate (PPi).
The probe DHC can coordinate with Cu2+ and consequently display on-off type fluorescence response.
Furthermore, the in situ formed nonfluorescent Cu2+-DHC complex can act as an effective off-on type fluorescent probe for sensing PPi due to the higher coordination reactivity between Cu2+ and PPi than that between Cu2+ and DHC.
The subsequent addition of PPase to the mixture containing Cu2+, DHC, and PPi leads to the fluorescence requenching of the system again (an off state) because PPase catalyzes the hydrolysis of PPi into orthophosphate in the reaction system.
Under the optimum conditions, the decrease of the fluorescence intensity of DHC-Cu2+-PPi system was linear with the increase of the PPase activity in the range from 0.1 to 0.3 U.
The detection limit was down to 0.028 U PPase (S/N=3).
Moreover, the as-established system was also applied to evaluate PPase inhibitor.
This study offers a simple yet effective method for the detection of PPase activity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhao, Lingzhi& Zhao, Liu& Miao, Yanqing& Liu, Chunye& Zhang, Chengxiao. 2016. Construction of a Turn Off-On-Off Fluorescent System Based on Competitive Coordination of Cu2+ between 6,7-Dihydroxycoumarin and Pyrophosphate Ion for Sensitive Assay of Pyrophosphatase Activity. Journal of Analytical Methods in Chemistry،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1107315
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhao, Lingzhi…[et al.]. Construction of a Turn Off-On-Off Fluorescent System Based on Competitive Coordination of Cu2+ between 6,7-Dihydroxycoumarin and Pyrophosphate Ion for Sensitive Assay of Pyrophosphatase Activity. Journal of Analytical Methods in Chemistry No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1107315
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhao, Lingzhi& Zhao, Liu& Miao, Yanqing& Liu, Chunye& Zhang, Chengxiao. Construction of a Turn Off-On-Off Fluorescent System Based on Competitive Coordination of Cu2+ between 6,7-Dihydroxycoumarin and Pyrophosphate Ion for Sensitive Assay of Pyrophosphatase Activity. Journal of Analytical Methods in Chemistry. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1107315
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1107315
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر