Construction of a Turn Off-On-Off Fluorescent System Based on Competitive Coordination of Cu2+ between 6,7-Dihydroxycoumarin and Pyrophosphate Ion for Sensitive Assay of Pyrophosphatase Activity
Joint Authors
Zhang, Chengxiao
Zhao, Lingzhi
Zhao, Liu
Miao, Yanqing
Liu, Chunye
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The detection of pyrophosphatase (PPase) activity is of great significance in diagnosing diseases and understanding the function of PPase-related biological events.
This study constructed a turn off-on-off fluorescent system for PPase activity assay based on PPase-regulated competitive coordination of Cu2+ between a water-soluble fluorescent probe 6,7-dihydroxycoumarin (DHC) and pyrophosphate (PPi).
The probe DHC can coordinate with Cu2+ and consequently display on-off type fluorescence response.
Furthermore, the in situ formed nonfluorescent Cu2+-DHC complex can act as an effective off-on type fluorescent probe for sensing PPi due to the higher coordination reactivity between Cu2+ and PPi than that between Cu2+ and DHC.
The subsequent addition of PPase to the mixture containing Cu2+, DHC, and PPi leads to the fluorescence requenching of the system again (an off state) because PPase catalyzes the hydrolysis of PPi into orthophosphate in the reaction system.
Under the optimum conditions, the decrease of the fluorescence intensity of DHC-Cu2+-PPi system was linear with the increase of the PPase activity in the range from 0.1 to 0.3 U.
The detection limit was down to 0.028 U PPase (S/N=3).
Moreover, the as-established system was also applied to evaluate PPase inhibitor.
This study offers a simple yet effective method for the detection of PPase activity.
American Psychological Association (APA)
Zhao, Lingzhi& Zhao, Liu& Miao, Yanqing& Liu, Chunye& Zhang, Chengxiao. 2016. Construction of a Turn Off-On-Off Fluorescent System Based on Competitive Coordination of Cu2+ between 6,7-Dihydroxycoumarin and Pyrophosphate Ion for Sensitive Assay of Pyrophosphatase Activity. Journal of Analytical Methods in Chemistry،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1107315
Modern Language Association (MLA)
Zhao, Lingzhi…[et al.]. Construction of a Turn Off-On-Off Fluorescent System Based on Competitive Coordination of Cu2+ between 6,7-Dihydroxycoumarin and Pyrophosphate Ion for Sensitive Assay of Pyrophosphatase Activity. Journal of Analytical Methods in Chemistry No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1107315
American Medical Association (AMA)
Zhao, Lingzhi& Zhao, Liu& Miao, Yanqing& Liu, Chunye& Zhang, Chengxiao. Construction of a Turn Off-On-Off Fluorescent System Based on Competitive Coordination of Cu2+ between 6,7-Dihydroxycoumarin and Pyrophosphate Ion for Sensitive Assay of Pyrophosphatase Activity. Journal of Analytical Methods in Chemistry. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1107315
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1107315