Quantitative Clinical Diagnostic Analysis of Acetone in Human Blood by HPLC: A Metabolomic Search for Acetone as Indicator
المؤلفون المشاركون
Akgul Kalkan, Esin
Sahiner, Mehtap
Ulker Cakir, Dilek
Alpaslan, Duygu
Yilmaz, Selehattin
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-05-19
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Using high-performance liquid chromatography (HPLC) and 2,4-dinitrophenylhydrazine (2,4-DNPH) as a derivatizing reagent, an analytical method was developed for the quantitative determination of acetone in human blood.
The determination was carried out at 365 nm using an ultraviolet-visible (UV-Vis) diode array detector (DAD).
For acetone as its 2,4-dinitrophenylhydrazone derivative, a good separation was achieved with a ThermoAcclaim C18 column (15 cm × 4.6 mm × 3 μm) at retention time (tR) 12.10 min and flowrate of 1 mL min−1 using a (methanol/acetonitrile) water elution gradient.
The methodology is simple, rapid, sensitive, and of low cost, exhibits good reproducibility, and allows the analysis of acetone in biological fluids.
A calibration curve was obtained for acetone using its standard solutions in acetonitrile.
Quantitative analysis of acetone in human blood was successfully carried out using this calibration graph.
The applied method was validated in parameters of linearity, limit of detection and quantification, accuracy, and precision.
We also present acetone as a useful tool for the HPLC-based metabolomic investigation of endogenous metabolism and quantitative clinical diagnostic analysis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Akgul Kalkan, Esin& Sahiner, Mehtap& Ulker Cakir, Dilek& Alpaslan, Duygu& Yilmaz, Selehattin. 2016. Quantitative Clinical Diagnostic Analysis of Acetone in Human Blood by HPLC: A Metabolomic Search for Acetone as Indicator. Journal of Analytical Methods in Chemistry،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1107334
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Akgul Kalkan, Esin…[et al.]. Quantitative Clinical Diagnostic Analysis of Acetone in Human Blood by HPLC: A Metabolomic Search for Acetone as Indicator. Journal of Analytical Methods in Chemistry No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1107334
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Akgul Kalkan, Esin& Sahiner, Mehtap& Ulker Cakir, Dilek& Alpaslan, Duygu& Yilmaz, Selehattin. Quantitative Clinical Diagnostic Analysis of Acetone in Human Blood by HPLC: A Metabolomic Search for Acetone as Indicator. Journal of Analytical Methods in Chemistry. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1107334
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1107334
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر