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Quantitative Clinical Diagnostic Analysis of Acetone in Human Blood by HPLC: A Metabolomic Search for Acetone as Indicator
Joint Authors
Akgul Kalkan, Esin
Sahiner, Mehtap
Ulker Cakir, Dilek
Alpaslan, Duygu
Yilmaz, Selehattin
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-19
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Using high-performance liquid chromatography (HPLC) and 2,4-dinitrophenylhydrazine (2,4-DNPH) as a derivatizing reagent, an analytical method was developed for the quantitative determination of acetone in human blood.
The determination was carried out at 365 nm using an ultraviolet-visible (UV-Vis) diode array detector (DAD).
For acetone as its 2,4-dinitrophenylhydrazone derivative, a good separation was achieved with a ThermoAcclaim C18 column (15 cm × 4.6 mm × 3 μm) at retention time (tR) 12.10 min and flowrate of 1 mL min−1 using a (methanol/acetonitrile) water elution gradient.
The methodology is simple, rapid, sensitive, and of low cost, exhibits good reproducibility, and allows the analysis of acetone in biological fluids.
A calibration curve was obtained for acetone using its standard solutions in acetonitrile.
Quantitative analysis of acetone in human blood was successfully carried out using this calibration graph.
The applied method was validated in parameters of linearity, limit of detection and quantification, accuracy, and precision.
We also present acetone as a useful tool for the HPLC-based metabolomic investigation of endogenous metabolism and quantitative clinical diagnostic analysis.
American Psychological Association (APA)
Akgul Kalkan, Esin& Sahiner, Mehtap& Ulker Cakir, Dilek& Alpaslan, Duygu& Yilmaz, Selehattin. 2016. Quantitative Clinical Diagnostic Analysis of Acetone in Human Blood by HPLC: A Metabolomic Search for Acetone as Indicator. Journal of Analytical Methods in Chemistry،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1107334
Modern Language Association (MLA)
Akgul Kalkan, Esin…[et al.]. Quantitative Clinical Diagnostic Analysis of Acetone in Human Blood by HPLC: A Metabolomic Search for Acetone as Indicator. Journal of Analytical Methods in Chemistry No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1107334
American Medical Association (AMA)
Akgul Kalkan, Esin& Sahiner, Mehtap& Ulker Cakir, Dilek& Alpaslan, Duygu& Yilmaz, Selehattin. Quantitative Clinical Diagnostic Analysis of Acetone in Human Blood by HPLC: A Metabolomic Search for Acetone as Indicator. Journal of Analytical Methods in Chemistry. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1107334
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1107334