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Determination of Levetiracetam in Human Plasma by Dispersive Liquid-Liquid Microextraction Followed by Gas Chromatography-Mass Spectrometry
المؤلفون المشاركون
de Moraes, Luiz Alberto Beraldo
de Gaitani, Cristiane Masetto
Alcantara, Greyce Kelly Steinhorst
Calixto, Leandro Augusto
Queiroz, Regina Helena Costa
Marquele-Oliveira, Franciane
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-10-17
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Levetiracetam (LEV) is an antiepileptic drug that is clinically effective in generalized and partial epilepsy syndromes.
The use of this drug has been increasing in clinical practice and intra- or -interindividual variability has been exhibited for special population.
For this reason, bioanalytical methods are required for drug monitoring in biological matrices.
So this work presents a dispersive liquid-liquid microextraction method followed by gas chromatography-mass spectrometry (DLLME-GC-MS) for LEV quantification in human plasma.
However, due to the matrix complexity a previous purification step is required.
Unlike other pretreatment techniques presented in the literature, for the first time, a procedure employing ultrafiltration tubes Amicon® (10 kDa porous size) without organic solvent consumption was developed.
GC-MS analyses were carried out using a linear temperature program, capillary fused silica column, and helium as the carrier gas.
DLLME optimized parameters were type and volume of extraction and dispersing solvents, salt addition, and vortex agitation time.
Under chosen parameters (extraction solvent: chloroform, 130 μL; dispersing solvent: isopropyl alcohol, 400 μL; no salt addition and no vortex agitation time), the method was completely validated and all parameters were in agreement with the literature recommendations.
LEV was quantified in patient’s plasma sample using less than 550 μL of organic solvent.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Alcantara, Greyce Kelly Steinhorst& Calixto, Leandro Augusto& de Moraes, Luiz Alberto Beraldo& Queiroz, Regina Helena Costa& Marquele-Oliveira, Franciane& de Gaitani, Cristiane Masetto. 2016. Determination of Levetiracetam in Human Plasma by Dispersive Liquid-Liquid Microextraction Followed by Gas Chromatography-Mass Spectrometry. Journal of Analytical Methods in Chemistry،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1107342
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Alcantara, Greyce Kelly Steinhorst…[et al.]. Determination of Levetiracetam in Human Plasma by Dispersive Liquid-Liquid Microextraction Followed by Gas Chromatography-Mass Spectrometry. Journal of Analytical Methods in Chemistry No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1107342
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Alcantara, Greyce Kelly Steinhorst& Calixto, Leandro Augusto& de Moraes, Luiz Alberto Beraldo& Queiroz, Regina Helena Costa& Marquele-Oliveira, Franciane& de Gaitani, Cristiane Masetto. Determination of Levetiracetam in Human Plasma by Dispersive Liquid-Liquid Microextraction Followed by Gas Chromatography-Mass Spectrometry. Journal of Analytical Methods in Chemistry. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1107342
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1107342
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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