Determination of Levetiracetam in Human Plasma by Dispersive Liquid-Liquid Microextraction Followed by Gas Chromatography-Mass Spectrometry
Joint Authors
de Moraes, Luiz Alberto Beraldo
de Gaitani, Cristiane Masetto
Alcantara, Greyce Kelly Steinhorst
Calixto, Leandro Augusto
Queiroz, Regina Helena Costa
Marquele-Oliveira, Franciane
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-17
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Levetiracetam (LEV) is an antiepileptic drug that is clinically effective in generalized and partial epilepsy syndromes.
The use of this drug has been increasing in clinical practice and intra- or -interindividual variability has been exhibited for special population.
For this reason, bioanalytical methods are required for drug monitoring in biological matrices.
So this work presents a dispersive liquid-liquid microextraction method followed by gas chromatography-mass spectrometry (DLLME-GC-MS) for LEV quantification in human plasma.
However, due to the matrix complexity a previous purification step is required.
Unlike other pretreatment techniques presented in the literature, for the first time, a procedure employing ultrafiltration tubes Amicon® (10 kDa porous size) without organic solvent consumption was developed.
GC-MS analyses were carried out using a linear temperature program, capillary fused silica column, and helium as the carrier gas.
DLLME optimized parameters were type and volume of extraction and dispersing solvents, salt addition, and vortex agitation time.
Under chosen parameters (extraction solvent: chloroform, 130 μL; dispersing solvent: isopropyl alcohol, 400 μL; no salt addition and no vortex agitation time), the method was completely validated and all parameters were in agreement with the literature recommendations.
LEV was quantified in patient’s plasma sample using less than 550 μL of organic solvent.
American Psychological Association (APA)
Alcantara, Greyce Kelly Steinhorst& Calixto, Leandro Augusto& de Moraes, Luiz Alberto Beraldo& Queiroz, Regina Helena Costa& Marquele-Oliveira, Franciane& de Gaitani, Cristiane Masetto. 2016. Determination of Levetiracetam in Human Plasma by Dispersive Liquid-Liquid Microextraction Followed by Gas Chromatography-Mass Spectrometry. Journal of Analytical Methods in Chemistry،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1107342
Modern Language Association (MLA)
Alcantara, Greyce Kelly Steinhorst…[et al.]. Determination of Levetiracetam in Human Plasma by Dispersive Liquid-Liquid Microextraction Followed by Gas Chromatography-Mass Spectrometry. Journal of Analytical Methods in Chemistry No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1107342
American Medical Association (AMA)
Alcantara, Greyce Kelly Steinhorst& Calixto, Leandro Augusto& de Moraes, Luiz Alberto Beraldo& Queiroz, Regina Helena Costa& Marquele-Oliveira, Franciane& de Gaitani, Cristiane Masetto. Determination of Levetiracetam in Human Plasma by Dispersive Liquid-Liquid Microextraction Followed by Gas Chromatography-Mass Spectrometry. Journal of Analytical Methods in Chemistry. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1107342
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1107342