Colloidal Gold Probe-Based Immunochromatographic Strip Assay for the Rapid Detection of Microbial Transglutaminase in Frozen Surimi

المؤلفون المشاركون

Zhao, Jianxin
Zhang, Hao
Fan, Daming
Li, Yi
Gu, Zhennan
Huang, Jianlian
Zhou, Wenguo
Zhang, Wenhai

المصدر

Journal of Chemistry

العدد

المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2016-08-24

دولة النشر

مصر

عدد الصفحات

7

التخصصات الرئيسية

الكيمياء

الملخص EN

Adding microbial transglutaminase (MTGase) to frozen surimi to enable the surimi to be sold as a higher-grade product at a higher price defrauds surimi product manufacturers and undercuts legitimate industry prices.

Therefore, it is important to develop an accurate method of detecting the presence of MTGase in surimi.

In this study, an immunochromatographic strip assay with a colloidal gold antibody probe was successfully developed and used to rapidly and qualitatively detect MTGase in surimi samples.

The results were obtained in less than 10 min.

The limit for the qualitative detection of MTGase using the immunochromatographic strip assay was identified as 1.0 μg/mL.

The results of the immunochromatographic strip analysis of frozen surimi samples were verified by comparison with the results of a sandwich enzyme-linked immunosorbent assay.

The colloidal gold probe-based immunochromatographic strip assay was thus found to be a rapid, economical, and user friendly method of detecting MTGase in surimi.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Fan, Daming& Li, Yi& Gu, Zhennan& Huang, Jianlian& Zhou, Wenguo& Zhang, Wenhai…[et al.]. 2016. Colloidal Gold Probe-Based Immunochromatographic Strip Assay for the Rapid Detection of Microbial Transglutaminase in Frozen Surimi. Journal of Chemistry،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1107787

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Fan, Daming…[et al.]. Colloidal Gold Probe-Based Immunochromatographic Strip Assay for the Rapid Detection of Microbial Transglutaminase in Frozen Surimi. Journal of Chemistry No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1107787

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Fan, Daming& Li, Yi& Gu, Zhennan& Huang, Jianlian& Zhou, Wenguo& Zhang, Wenhai…[et al.]. Colloidal Gold Probe-Based Immunochromatographic Strip Assay for the Rapid Detection of Microbial Transglutaminase in Frozen Surimi. Journal of Chemistry. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1107787

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1107787