Colloidal Gold Probe-Based Immunochromatographic Strip Assay for the Rapid Detection of Microbial Transglutaminase in Frozen Surimi
Joint Authors
Zhao, Jianxin
Zhang, Hao
Fan, Daming
Li, Yi
Gu, Zhennan
Huang, Jianlian
Zhou, Wenguo
Zhang, Wenhai
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Adding microbial transglutaminase (MTGase) to frozen surimi to enable the surimi to be sold as a higher-grade product at a higher price defrauds surimi product manufacturers and undercuts legitimate industry prices.
Therefore, it is important to develop an accurate method of detecting the presence of MTGase in surimi.
In this study, an immunochromatographic strip assay with a colloidal gold antibody probe was successfully developed and used to rapidly and qualitatively detect MTGase in surimi samples.
The results were obtained in less than 10 min.
The limit for the qualitative detection of MTGase using the immunochromatographic strip assay was identified as 1.0 μg/mL.
The results of the immunochromatographic strip analysis of frozen surimi samples were verified by comparison with the results of a sandwich enzyme-linked immunosorbent assay.
The colloidal gold probe-based immunochromatographic strip assay was thus found to be a rapid, economical, and user friendly method of detecting MTGase in surimi.
American Psychological Association (APA)
Fan, Daming& Li, Yi& Gu, Zhennan& Huang, Jianlian& Zhou, Wenguo& Zhang, Wenhai…[et al.]. 2016. Colloidal Gold Probe-Based Immunochromatographic Strip Assay for the Rapid Detection of Microbial Transglutaminase in Frozen Surimi. Journal of Chemistry،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1107787
Modern Language Association (MLA)
Fan, Daming…[et al.]. Colloidal Gold Probe-Based Immunochromatographic Strip Assay for the Rapid Detection of Microbial Transglutaminase in Frozen Surimi. Journal of Chemistry No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1107787
American Medical Association (AMA)
Fan, Daming& Li, Yi& Gu, Zhennan& Huang, Jianlian& Zhou, Wenguo& Zhang, Wenhai…[et al.]. Colloidal Gold Probe-Based Immunochromatographic Strip Assay for the Rapid Detection of Microbial Transglutaminase in Frozen Surimi. Journal of Chemistry. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1107787
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1107787