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Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells
المؤلفون المشاركون
Barati, Michelle T.
Gould, James C.
Salyer, Sarah A.
Isaacs, Susan
Wilkey, Daniel W.
Merchant, Michael L.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-12-29
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
The effects of acute exposure to high glucose levels as experienced by glomerular mesangial cells in postprandial conditions and states such as in prediabetes were investigated using proteomic methods.
Two-dimensional gel electrophoresis and matrix assisted laser desorption ionization time of flight mass spectrometry methods were used to identify protein expression patterns in immortalized rat mesangial cells altered by 2 h high glucose (HG) growth conditions as compared to isoosmotic/normal glucose control (NG⁎) conditions.
Unique protein expression changes at 2 h HG treatment were measured for 51 protein spots.
These proteins could be broadly grouped into two categories: (1) proteins involved in cell survival/cell signaling and (2) proteins involved in stress response.
Immunoblot experiments for a protein belonging to both categories, prohibitin (PHB), supported a trend for increased total expression as well as significant increases in an acidic PHB isoform.
Additional studies confirmed the regulation of proteasomal subunit alpha-type 2 and the endoplasmic reticulum chaperone and oxidoreductase PDI (protein disulfide isomerase), suggesting altered ER protein folding capacity and proteasomal function in response to acute HG.
We conclude that short term high glucose induces subtle changes in protein abundances suggesting posttranslational modifications and regulation of pathways involved in proteostasis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Barati, Michelle T.& Gould, James C.& Salyer, Sarah A.& Isaacs, Susan& Wilkey, Daniel W.& Merchant, Michael L.. 2015. Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1108066
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Barati, Michelle T.…[et al.]. Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells. Journal of Diabetes Research No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1108066
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Barati, Michelle T.& Gould, James C.& Salyer, Sarah A.& Isaacs, Susan& Wilkey, Daniel W.& Merchant, Michael L.. Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells. Journal of Diabetes Research. 2015. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1108066
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1108066
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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