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Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells
Joint Authors
Barati, Michelle T.
Gould, James C.
Salyer, Sarah A.
Isaacs, Susan
Wilkey, Daniel W.
Merchant, Michael L.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-29
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The effects of acute exposure to high glucose levels as experienced by glomerular mesangial cells in postprandial conditions and states such as in prediabetes were investigated using proteomic methods.
Two-dimensional gel electrophoresis and matrix assisted laser desorption ionization time of flight mass spectrometry methods were used to identify protein expression patterns in immortalized rat mesangial cells altered by 2 h high glucose (HG) growth conditions as compared to isoosmotic/normal glucose control (NG⁎) conditions.
Unique protein expression changes at 2 h HG treatment were measured for 51 protein spots.
These proteins could be broadly grouped into two categories: (1) proteins involved in cell survival/cell signaling and (2) proteins involved in stress response.
Immunoblot experiments for a protein belonging to both categories, prohibitin (PHB), supported a trend for increased total expression as well as significant increases in an acidic PHB isoform.
Additional studies confirmed the regulation of proteasomal subunit alpha-type 2 and the endoplasmic reticulum chaperone and oxidoreductase PDI (protein disulfide isomerase), suggesting altered ER protein folding capacity and proteasomal function in response to acute HG.
We conclude that short term high glucose induces subtle changes in protein abundances suggesting posttranslational modifications and regulation of pathways involved in proteostasis.
American Psychological Association (APA)
Barati, Michelle T.& Gould, James C.& Salyer, Sarah A.& Isaacs, Susan& Wilkey, Daniel W.& Merchant, Michael L.. 2015. Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1108066
Modern Language Association (MLA)
Barati, Michelle T.…[et al.]. Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells. Journal of Diabetes Research No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1108066
American Medical Association (AMA)
Barati, Michelle T.& Gould, James C.& Salyer, Sarah A.& Isaacs, Susan& Wilkey, Daniel W.& Merchant, Michael L.. Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells. Journal of Diabetes Research. 2015. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1108066
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108066