Continuous Quadrupole Magnetic Separation of Islets during Digestion Improves Purified Porcine Islet Viability
المؤلفون المشاركون
Weegman, Bradley P.
Kumar Sajja, Venkata Sunil
Suszynski, Thomas M.
Rizzari, Michael D.
Scott III, William E.
Kitzmann, Jennifer P.
Mueller, Kate R.
Hanley, Thomas R.
Kennedy, David J.
Todd, Paul W.
Balamurugan, Appakalai N.
Hering, Bernhard J.
Papas, Klearchos K.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-10-23
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Islet transplantation (ITx) is an emerging and promising therapy for patients with uncontrolled type 1 diabetes.
The islet isolation and purification processes require exposure to extended cold ischemia, warm-enzymatic digestion, mechanical agitation, and use of damaging chemicals for density gradient separation (DG), all of which reduce viable islet yield.
In this paper, we describe initial proof-of-concept studies exploring quadrupole magnetic separation (QMS) of islets as an alternative to DG to reduce exposure to these harsh conditions.
Three porcine pancreata were split into two parts, the splenic lobe (SPL) and the combined connecting/duodenal lobes (CDL), for paired digestions and purifications.
Islets in the SPL were preferentially labeled using magnetic microparticles (MMPs) that lodge within the islet microvasculature when infused into the pancreas and were continuously separated from the exocrine tissue by QMS during the collection phase of the digestion process.
Unlabeled islets from the CDL were purified by conventional DG.
Islets purified by QMS exhibited significantly improved viability (measured by oxygen consumption rate per DNA, p<0.03) and better morphology relative to control islets.
Islet purification by QMS can reduce the detrimental effects of prolonged exposure to toxic enzymes and density gradient solutions and substantially improve islet viability after isolation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Weegman, Bradley P.& Kumar Sajja, Venkata Sunil& Suszynski, Thomas M.& Rizzari, Michael D.& Scott III, William E.& Kitzmann, Jennifer P.…[et al.]. 2016. Continuous Quadrupole Magnetic Separation of Islets during Digestion Improves Purified Porcine Islet Viability. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108188
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Weegman, Bradley P.…[et al.]. Continuous Quadrupole Magnetic Separation of Islets during Digestion Improves Purified Porcine Islet Viability. Journal of Diabetes Research No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1108188
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Weegman, Bradley P.& Kumar Sajja, Venkata Sunil& Suszynski, Thomas M.& Rizzari, Michael D.& Scott III, William E.& Kitzmann, Jennifer P.…[et al.]. Continuous Quadrupole Magnetic Separation of Islets during Digestion Improves Purified Porcine Islet Viability. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108188
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1108188
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر