Continuous Quadrupole Magnetic Separation of Islets during Digestion Improves Purified Porcine Islet Viability
Joint Authors
Weegman, Bradley P.
Kumar Sajja, Venkata Sunil
Suszynski, Thomas M.
Rizzari, Michael D.
Scott III, William E.
Kitzmann, Jennifer P.
Mueller, Kate R.
Hanley, Thomas R.
Kennedy, David J.
Todd, Paul W.
Balamurugan, Appakalai N.
Hering, Bernhard J.
Papas, Klearchos K.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Islet transplantation (ITx) is an emerging and promising therapy for patients with uncontrolled type 1 diabetes.
The islet isolation and purification processes require exposure to extended cold ischemia, warm-enzymatic digestion, mechanical agitation, and use of damaging chemicals for density gradient separation (DG), all of which reduce viable islet yield.
In this paper, we describe initial proof-of-concept studies exploring quadrupole magnetic separation (QMS) of islets as an alternative to DG to reduce exposure to these harsh conditions.
Three porcine pancreata were split into two parts, the splenic lobe (SPL) and the combined connecting/duodenal lobes (CDL), for paired digestions and purifications.
Islets in the SPL were preferentially labeled using magnetic microparticles (MMPs) that lodge within the islet microvasculature when infused into the pancreas and were continuously separated from the exocrine tissue by QMS during the collection phase of the digestion process.
Unlabeled islets from the CDL were purified by conventional DG.
Islets purified by QMS exhibited significantly improved viability (measured by oxygen consumption rate per DNA, p<0.03) and better morphology relative to control islets.
Islet purification by QMS can reduce the detrimental effects of prolonged exposure to toxic enzymes and density gradient solutions and substantially improve islet viability after isolation.
American Psychological Association (APA)
Weegman, Bradley P.& Kumar Sajja, Venkata Sunil& Suszynski, Thomas M.& Rizzari, Michael D.& Scott III, William E.& Kitzmann, Jennifer P.…[et al.]. 2016. Continuous Quadrupole Magnetic Separation of Islets during Digestion Improves Purified Porcine Islet Viability. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108188
Modern Language Association (MLA)
Weegman, Bradley P.…[et al.]. Continuous Quadrupole Magnetic Separation of Islets during Digestion Improves Purified Porcine Islet Viability. Journal of Diabetes Research No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1108188
American Medical Association (AMA)
Weegman, Bradley P.& Kumar Sajja, Venkata Sunil& Suszynski, Thomas M.& Rizzari, Michael D.& Scott III, William E.& Kitzmann, Jennifer P.…[et al.]. Continuous Quadrupole Magnetic Separation of Islets during Digestion Improves Purified Porcine Islet Viability. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108188
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108188