A Fusion Protein Consisting of the Vaccine Adjuvant Monophosphoryl Lipid A and the Allergen Ovalbumin Boosts Allergen-Specific Th1, Th2, and Th17 Responses In Vitro
المؤلفون المشاركون
Schülke, Stefan
Vogel, Lothar
Junker, Ann-Christine
Hanschmann, Kay-Martin
Flaczyk, Adam
Vieths, Stefan
Scheurer, Stephan
المصدر
Journal of Immunology Research
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-06-01
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Background.
The detoxified TLR4-ligand Monophosphoryl Lipid A (MPLA) is the first approved TLR-agonist used as adjuvant in licensed vaccines but has not yet been explored as part of conjugated vaccines.
Objective.
To investigate the immune-modulating properties of a fusion protein consisting of MPLA and Ovalbumin (MPLA : Ova).
Results.
MPLA and Ova were chemically coupled by stable carbamate linkage.
MPLA : Ova was highly pure without detectable product-related impurities by either noncoupled MPLA or Ova.
Light scattering analysis revealed MPLA : Ova to be aggregated.
Stimulation of mDC and mDC : DO11.10 CD4+ TC cocultures showed a stronger activation of both mDC and Ova-specific DO11.10 CD4+ TC by MPLA : Ova compared to the mixture of both components.
MPLA : Ova induced both strong proinflammatory (IL-1β, IL-6, and TNF-α) and anti-inflammatory (IL-10) cytokine responses from mDCs while also boosting allergen-specific Th1, Th2, and Th17 cytokine secretion.
Conclusion.
Conjugation of MPLA and antigen enhanced the immune response compared to the mixture of both components.
Due to the nonbiased boost of Ova-specific Th2 and Th17 responses while also inducing Th1 responses, this fusion protein may not be a suitable vaccine candidate for allergy treatment but may hold potential for the treatment of other diseases that require a strong stimulation of the host’s immune system (e.g., cancer).
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Schülke, Stefan& Vogel, Lothar& Junker, Ann-Christine& Hanschmann, Kay-Martin& Flaczyk, Adam& Vieths, Stefan…[et al.]. 2016. A Fusion Protein Consisting of the Vaccine Adjuvant Monophosphoryl Lipid A and the Allergen Ovalbumin Boosts Allergen-Specific Th1, Th2, and Th17 Responses In Vitro. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1108780
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Schülke, Stefan…[et al.]. A Fusion Protein Consisting of the Vaccine Adjuvant Monophosphoryl Lipid A and the Allergen Ovalbumin Boosts Allergen-Specific Th1, Th2, and Th17 Responses In Vitro. Journal of Immunology Research No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1108780
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Schülke, Stefan& Vogel, Lothar& Junker, Ann-Christine& Hanschmann, Kay-Martin& Flaczyk, Adam& Vieths, Stefan…[et al.]. A Fusion Protein Consisting of the Vaccine Adjuvant Monophosphoryl Lipid A and the Allergen Ovalbumin Boosts Allergen-Specific Th1, Th2, and Th17 Responses In Vitro. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1108780
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1108780
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر