A Fusion Protein Consisting of the Vaccine Adjuvant Monophosphoryl Lipid A and the Allergen Ovalbumin Boosts Allergen-Specific Th1, Th2, and Th17 Responses In Vitro
Joint Authors
Schülke, Stefan
Vogel, Lothar
Junker, Ann-Christine
Hanschmann, Kay-Martin
Flaczyk, Adam
Vieths, Stefan
Scheurer, Stephan
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
The detoxified TLR4-ligand Monophosphoryl Lipid A (MPLA) is the first approved TLR-agonist used as adjuvant in licensed vaccines but has not yet been explored as part of conjugated vaccines.
Objective.
To investigate the immune-modulating properties of a fusion protein consisting of MPLA and Ovalbumin (MPLA : Ova).
Results.
MPLA and Ova were chemically coupled by stable carbamate linkage.
MPLA : Ova was highly pure without detectable product-related impurities by either noncoupled MPLA or Ova.
Light scattering analysis revealed MPLA : Ova to be aggregated.
Stimulation of mDC and mDC : DO11.10 CD4+ TC cocultures showed a stronger activation of both mDC and Ova-specific DO11.10 CD4+ TC by MPLA : Ova compared to the mixture of both components.
MPLA : Ova induced both strong proinflammatory (IL-1β, IL-6, and TNF-α) and anti-inflammatory (IL-10) cytokine responses from mDCs while also boosting allergen-specific Th1, Th2, and Th17 cytokine secretion.
Conclusion.
Conjugation of MPLA and antigen enhanced the immune response compared to the mixture of both components.
Due to the nonbiased boost of Ova-specific Th2 and Th17 responses while also inducing Th1 responses, this fusion protein may not be a suitable vaccine candidate for allergy treatment but may hold potential for the treatment of other diseases that require a strong stimulation of the host’s immune system (e.g., cancer).
American Psychological Association (APA)
Schülke, Stefan& Vogel, Lothar& Junker, Ann-Christine& Hanschmann, Kay-Martin& Flaczyk, Adam& Vieths, Stefan…[et al.]. 2016. A Fusion Protein Consisting of the Vaccine Adjuvant Monophosphoryl Lipid A and the Allergen Ovalbumin Boosts Allergen-Specific Th1, Th2, and Th17 Responses In Vitro. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1108780
Modern Language Association (MLA)
Schülke, Stefan…[et al.]. A Fusion Protein Consisting of the Vaccine Adjuvant Monophosphoryl Lipid A and the Allergen Ovalbumin Boosts Allergen-Specific Th1, Th2, and Th17 Responses In Vitro. Journal of Immunology Research No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1108780
American Medical Association (AMA)
Schülke, Stefan& Vogel, Lothar& Junker, Ann-Christine& Hanschmann, Kay-Martin& Flaczyk, Adam& Vieths, Stefan…[et al.]. A Fusion Protein Consisting of the Vaccine Adjuvant Monophosphoryl Lipid A and the Allergen Ovalbumin Boosts Allergen-Specific Th1, Th2, and Th17 Responses In Vitro. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1108780
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108780