Effects of Multiwalled Carbon Nanotube Surface Modification and Purification on Bovine Serum Albumin Binding and Biological Responses
المؤلفون المشاركون
Brown, Jared M.
Bai, Wei
Wu, Zheqiong
Mitra, Somenath
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-10-24
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Carboxylation of multiwalled carbon nanotubes (MWCNTs) has been used to improve solubility in aqueous systems and for further functionalization with biologically active moieties for biomedical uses.
An important consideration is that oxidation debris is generated during the process of carboxylation, which can be removed by base washing.
We hypothesized that surface modification as well as purification by debris removal may alter physicochemical properties of MWCNTs and their ability to bind proteins.
We utilized pristine MWCNT, carboxylated MWCNTs (F-MWCNTs), and base-washed carboxylated MWCNTs (BW-F-MWCNTs) to examine formation of a bovine serum albumin (BSA) protein corona and impact on biological responses.
We found that carboxylation increased the capability of F-MWCNTs to bind BSA, and base washing further increased this binding.
Functionalization increased cellular uptake by rat aortic endothelial cells (RAEC) and mouse macrophages (RAW264.7), while base washing showed results similar to the functionalized analog.
Interestingly, BSA binding downregulated mRNA levels of interleukin-6 (IL-6) and heme oxygenase 1 (Hmox1) in RAEC cells but upregulated the expression of IL-6 and Hmox1 in RAW264.7 cells.
Overall, our study demonstrated that surface modification as well as further purification impacted the interaction of MWCNTs with proteins and subsequent cellular responses.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bai, Wei& Wu, Zheqiong& Mitra, Somenath& Brown, Jared M.. 2016. Effects of Multiwalled Carbon Nanotube Surface Modification and Purification on Bovine Serum Albumin Binding and Biological Responses. Journal of Nanomaterials،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1109045
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bai, Wei…[et al.]. Effects of Multiwalled Carbon Nanotube Surface Modification and Purification on Bovine Serum Albumin Binding and Biological Responses. Journal of Nanomaterials No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1109045
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bai, Wei& Wu, Zheqiong& Mitra, Somenath& Brown, Jared M.. Effects of Multiwalled Carbon Nanotube Surface Modification and Purification on Bovine Serum Albumin Binding and Biological Responses. Journal of Nanomaterials. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1109045
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1109045
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر