Effects of Multiwalled Carbon Nanotube Surface Modification and Purification on Bovine Serum Albumin Binding and Biological Responses
Joint Authors
Brown, Jared M.
Bai, Wei
Wu, Zheqiong
Mitra, Somenath
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-24
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Carboxylation of multiwalled carbon nanotubes (MWCNTs) has been used to improve solubility in aqueous systems and for further functionalization with biologically active moieties for biomedical uses.
An important consideration is that oxidation debris is generated during the process of carboxylation, which can be removed by base washing.
We hypothesized that surface modification as well as purification by debris removal may alter physicochemical properties of MWCNTs and their ability to bind proteins.
We utilized pristine MWCNT, carboxylated MWCNTs (F-MWCNTs), and base-washed carboxylated MWCNTs (BW-F-MWCNTs) to examine formation of a bovine serum albumin (BSA) protein corona and impact on biological responses.
We found that carboxylation increased the capability of F-MWCNTs to bind BSA, and base washing further increased this binding.
Functionalization increased cellular uptake by rat aortic endothelial cells (RAEC) and mouse macrophages (RAW264.7), while base washing showed results similar to the functionalized analog.
Interestingly, BSA binding downregulated mRNA levels of interleukin-6 (IL-6) and heme oxygenase 1 (Hmox1) in RAEC cells but upregulated the expression of IL-6 and Hmox1 in RAW264.7 cells.
Overall, our study demonstrated that surface modification as well as further purification impacted the interaction of MWCNTs with proteins and subsequent cellular responses.
American Psychological Association (APA)
Bai, Wei& Wu, Zheqiong& Mitra, Somenath& Brown, Jared M.. 2016. Effects of Multiwalled Carbon Nanotube Surface Modification and Purification on Bovine Serum Albumin Binding and Biological Responses. Journal of Nanomaterials،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1109045
Modern Language Association (MLA)
Bai, Wei…[et al.]. Effects of Multiwalled Carbon Nanotube Surface Modification and Purification on Bovine Serum Albumin Binding and Biological Responses. Journal of Nanomaterials No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1109045
American Medical Association (AMA)
Bai, Wei& Wu, Zheqiong& Mitra, Somenath& Brown, Jared M.. Effects of Multiwalled Carbon Nanotube Surface Modification and Purification on Bovine Serum Albumin Binding and Biological Responses. Journal of Nanomaterials. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1109045
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1109045