A Novel and Disposable Enzyme-Labeled Amperometric Immunosensor Based on MWCNT Fibers for Listeria monocytogenes Detection
المؤلفون المشاركون
Li, Li
Lu, Ying
Liu, Yongling
Zhao, Yong
Li, Wenjiao
Qiu, Longbing
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-06-13
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
A sensitive and specific immunosensor was developed by immobilizing HRP-labeled antibody against Listeria monocytogenes onto the surface of the novel multiwalled carbon nanotube fibers.
Firstly, the influence of immunoelectrode modification methods (chemical and physical method) on detection sensitivity and stability was studied.
Next, immunosensor was developed, optimized, and applied for the detection of L.
monocytogenes.
The morphology was characterized by scanning electron microscopy (SEM), and electrochemical behaviors were by cyclic voltammetry.
SEM image, relative response (%), and current data showed chemical modification for immunoelectrode was helpful to capture more target bacteria and obtain more stable current response, resulting in improving the detection sensitivity.
The linear relationship between L.
monocytogenes concentration and Δ I p c was from 102 to 105 cfu/mL ( R 2 = 0.993 ), and LOD was 1.07 × 10 2 cfu/mL.
L.
monocytogenes in mixed bacteria (1.51 × 103 cfu/mL) of milk sample (S/N > 14) were detected by developed immunosensor, showing good specificity.
Good storage stability and reproducibility (RSD < 6.5%) also showed the potential application of immunosensor for the rapid detection of L.
monocytogenes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lu, Ying& Liu, Yongling& Zhao, Yong& Li, Wenjiao& Qiu, Longbing& Li, Li. 2016. A Novel and Disposable Enzyme-Labeled Amperometric Immunosensor Based on MWCNT Fibers for Listeria monocytogenes Detection. Journal of Nanomaterials،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1109135
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lu, Ying…[et al.]. A Novel and Disposable Enzyme-Labeled Amperometric Immunosensor Based on MWCNT Fibers for Listeria monocytogenes Detection. Journal of Nanomaterials No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1109135
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lu, Ying& Liu, Yongling& Zhao, Yong& Li, Wenjiao& Qiu, Longbing& Li, Li. A Novel and Disposable Enzyme-Labeled Amperometric Immunosensor Based on MWCNT Fibers for Listeria monocytogenes Detection. Journal of Nanomaterials. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1109135
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1109135
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر