A Novel and Disposable Enzyme-Labeled Amperometric Immunosensor Based on MWCNT Fibers for Listeria monocytogenes Detection
Joint Authors
Li, Li
Lu, Ying
Liu, Yongling
Zhao, Yong
Li, Wenjiao
Qiu, Longbing
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-13
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A sensitive and specific immunosensor was developed by immobilizing HRP-labeled antibody against Listeria monocytogenes onto the surface of the novel multiwalled carbon nanotube fibers.
Firstly, the influence of immunoelectrode modification methods (chemical and physical method) on detection sensitivity and stability was studied.
Next, immunosensor was developed, optimized, and applied for the detection of L.
monocytogenes.
The morphology was characterized by scanning electron microscopy (SEM), and electrochemical behaviors were by cyclic voltammetry.
SEM image, relative response (%), and current data showed chemical modification for immunoelectrode was helpful to capture more target bacteria and obtain more stable current response, resulting in improving the detection sensitivity.
The linear relationship between L.
monocytogenes concentration and Δ I p c was from 102 to 105 cfu/mL ( R 2 = 0.993 ), and LOD was 1.07 × 10 2 cfu/mL.
L.
monocytogenes in mixed bacteria (1.51 × 103 cfu/mL) of milk sample (S/N > 14) were detected by developed immunosensor, showing good specificity.
Good storage stability and reproducibility (RSD < 6.5%) also showed the potential application of immunosensor for the rapid detection of L.
monocytogenes.
American Psychological Association (APA)
Lu, Ying& Liu, Yongling& Zhao, Yong& Li, Wenjiao& Qiu, Longbing& Li, Li. 2016. A Novel and Disposable Enzyme-Labeled Amperometric Immunosensor Based on MWCNT Fibers for Listeria monocytogenes Detection. Journal of Nanomaterials،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1109135
Modern Language Association (MLA)
Lu, Ying…[et al.]. A Novel and Disposable Enzyme-Labeled Amperometric Immunosensor Based on MWCNT Fibers for Listeria monocytogenes Detection. Journal of Nanomaterials No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1109135
American Medical Association (AMA)
Lu, Ying& Liu, Yongling& Zhao, Yong& Li, Wenjiao& Qiu, Longbing& Li, Li. A Novel and Disposable Enzyme-Labeled Amperometric Immunosensor Based on MWCNT Fibers for Listeria monocytogenes Detection. Journal of Nanomaterials. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1109135
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1109135