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Binding Affinity, Cellular Uptake, and Subsequent Intracellular Trafficking of the Nano-Gene Vector P123-PEI-R13
المؤلفون المشاركون
Zhang, Yaguang
Shu, Hongmei
Hu, Jing
Zhang, Min
Wu, Junweng
Liu, Kehai
Zhu, Qing
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-12-12
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
A nano-gene vector PEI-P123-R13 was synthesized by cross-linking low molecular weight PEI with P123 and further coupling bifunctional peptide R13 to the polymer for targeting tumor and increasing cellular uptake.
The binding assessment of R13 to αvβ3 positive cells was performed by HRP labeling.
The internalization pathways of P123-PEI-R13/DNA complexes were investigated based on the effect of specific endocytic inhibitors on transfection efficiency.
The mechanism of intracellular trafficking was investigated based on the effect of endosome-lysosome acidification inhibitors, cytoskeleton, and dynein inhibitors on transfection efficiency.
The results indicated that the bifunctional peptide R13 had the ability of binding to αvβ3 positive cells in vitro.
The modification of P123-PEI-R13 with R13 made it display new property of internalization.
P123-PEI-R13/DNA complexes were conducted simultaneously via clathrin-mediated endocytosis, caveolin-mediated endocytosis, macropinocytosis, and possible energy-independent route.
After internalization, P123-PEI-R13/DNA complexes could escape from the endosome-lysosome system because of its acidification and further took microtubule as the track and dynein as the dynamic source to be transported toward the microtubule (+) end, to wit nucleus, under the action of microfilament, and with the aid of intermediate filament.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Yaguang& Shu, Hongmei& Hu, Jing& Zhang, Min& Wu, Junweng& Liu, Kehai…[et al.]. 2016. Binding Affinity, Cellular Uptake, and Subsequent Intracellular Trafficking of the Nano-Gene Vector P123-PEI-R13. Journal of Nanomaterials،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1109335
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Yaguang…[et al.]. Binding Affinity, Cellular Uptake, and Subsequent Intracellular Trafficking of the Nano-Gene Vector P123-PEI-R13. Journal of Nanomaterials No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1109335
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Yaguang& Shu, Hongmei& Hu, Jing& Zhang, Min& Wu, Junweng& Liu, Kehai…[et al.]. Binding Affinity, Cellular Uptake, and Subsequent Intracellular Trafficking of the Nano-Gene Vector P123-PEI-R13. Journal of Nanomaterials. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1109335
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1109335
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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