Binding Affinity, Cellular Uptake, and Subsequent Intracellular Trafficking of the Nano-Gene Vector P123-PEI-R13
Joint Authors
Zhang, Yaguang
Shu, Hongmei
Hu, Jing
Zhang, Min
Wu, Junweng
Liu, Kehai
Zhu, Qing
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-12
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A nano-gene vector PEI-P123-R13 was synthesized by cross-linking low molecular weight PEI with P123 and further coupling bifunctional peptide R13 to the polymer for targeting tumor and increasing cellular uptake.
The binding assessment of R13 to αvβ3 positive cells was performed by HRP labeling.
The internalization pathways of P123-PEI-R13/DNA complexes were investigated based on the effect of specific endocytic inhibitors on transfection efficiency.
The mechanism of intracellular trafficking was investigated based on the effect of endosome-lysosome acidification inhibitors, cytoskeleton, and dynein inhibitors on transfection efficiency.
The results indicated that the bifunctional peptide R13 had the ability of binding to αvβ3 positive cells in vitro.
The modification of P123-PEI-R13 with R13 made it display new property of internalization.
P123-PEI-R13/DNA complexes were conducted simultaneously via clathrin-mediated endocytosis, caveolin-mediated endocytosis, macropinocytosis, and possible energy-independent route.
After internalization, P123-PEI-R13/DNA complexes could escape from the endosome-lysosome system because of its acidification and further took microtubule as the track and dynein as the dynamic source to be transported toward the microtubule (+) end, to wit nucleus, under the action of microfilament, and with the aid of intermediate filament.
American Psychological Association (APA)
Zhang, Yaguang& Shu, Hongmei& Hu, Jing& Zhang, Min& Wu, Junweng& Liu, Kehai…[et al.]. 2016. Binding Affinity, Cellular Uptake, and Subsequent Intracellular Trafficking of the Nano-Gene Vector P123-PEI-R13. Journal of Nanomaterials،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1109335
Modern Language Association (MLA)
Zhang, Yaguang…[et al.]. Binding Affinity, Cellular Uptake, and Subsequent Intracellular Trafficking of the Nano-Gene Vector P123-PEI-R13. Journal of Nanomaterials No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1109335
American Medical Association (AMA)
Zhang, Yaguang& Shu, Hongmei& Hu, Jing& Zhang, Min& Wu, Junweng& Liu, Kehai…[et al.]. Binding Affinity, Cellular Uptake, and Subsequent Intracellular Trafficking of the Nano-Gene Vector P123-PEI-R13. Journal of Nanomaterials. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1109335
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1109335