Functionalized Solid-Sphere PEG-b-PCL Nanoparticles to Target Brain Capillary Endothelial Cells In Vitro
المؤلفون المشاركون
Grossen, Philip
Québatte, Gabriela
Witzigmann, Dominik
Prescianotto-Baschong, Cristina
Dieu, Le-Ha
Huwyler, Jörg
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-06-12
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Nanoparticles are increasingly used to implement drug targeting strategies.
In the present study, solid-sphere nanoparticles (SNPs) made of poly(ethylene glycol)-b-poly(ε-caprolactone) (PEG-b-PCL) were covalently linked to a monoclonal antibody (83-14 mAb) targeted against the human insulin receptor that is highly expressed on human brain microvascular endothelial cells.
Resulting targeted SNPs were characterized using transmission electron microscopy (TEM), cryo-TEM, dynamic light scattering, and fluorescence correlation spectroscopy.
The critical aggregation concentration was determined using a fluorescence approach.
Interaction with a well-characterized human in vitro model of the blood-brain barrier (hCMEC/D3) was analysed using an array of methods (flow cytometry, confocal laser scanning microscopy, and TEM).
The toxicity on hCMEC/D3 cells and in addition on the human liver cell line HepG2 was assessed using the MTT assay.
SNPs with a diameter of 80 nm and a homogeneous size distribution were obtained.
Successful conjugation of 83-14 mAb using a heterobifunctional linker resulted in 5-6 molecules of fluorescently labeled 83-14 mAb per SNP.
Functionalized SNPs were taken up by hCMEC/D3 cells efficiently without showing a significant toxic effect on cells of the blood-brain barrier and HepG2 cells.
These results indicate that functionalized PEG-b-PCL SNPs are a promising candidate to deliver drugs to the CNS.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Grossen, Philip& Québatte, Gabriela& Witzigmann, Dominik& Prescianotto-Baschong, Cristina& Dieu, Le-Ha& Huwyler, Jörg. 2016. Functionalized Solid-Sphere PEG-b-PCL Nanoparticles to Target Brain Capillary Endothelial Cells In Vitro. Journal of Nanomaterials،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1109382
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Grossen, Philip…[et al.]. Functionalized Solid-Sphere PEG-b-PCL Nanoparticles to Target Brain Capillary Endothelial Cells In Vitro. Journal of Nanomaterials No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1109382
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Grossen, Philip& Québatte, Gabriela& Witzigmann, Dominik& Prescianotto-Baschong, Cristina& Dieu, Le-Ha& Huwyler, Jörg. Functionalized Solid-Sphere PEG-b-PCL Nanoparticles to Target Brain Capillary Endothelial Cells In Vitro. Journal of Nanomaterials. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1109382
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1109382
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر