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Functionalized Solid-Sphere PEG-b-PCL Nanoparticles to Target Brain Capillary Endothelial Cells In Vitro
Joint Authors
Grossen, Philip
Québatte, Gabriela
Witzigmann, Dominik
Prescianotto-Baschong, Cristina
Dieu, Le-Ha
Huwyler, Jörg
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-12
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Nanoparticles are increasingly used to implement drug targeting strategies.
In the present study, solid-sphere nanoparticles (SNPs) made of poly(ethylene glycol)-b-poly(ε-caprolactone) (PEG-b-PCL) were covalently linked to a monoclonal antibody (83-14 mAb) targeted against the human insulin receptor that is highly expressed on human brain microvascular endothelial cells.
Resulting targeted SNPs were characterized using transmission electron microscopy (TEM), cryo-TEM, dynamic light scattering, and fluorescence correlation spectroscopy.
The critical aggregation concentration was determined using a fluorescence approach.
Interaction with a well-characterized human in vitro model of the blood-brain barrier (hCMEC/D3) was analysed using an array of methods (flow cytometry, confocal laser scanning microscopy, and TEM).
The toxicity on hCMEC/D3 cells and in addition on the human liver cell line HepG2 was assessed using the MTT assay.
SNPs with a diameter of 80 nm and a homogeneous size distribution were obtained.
Successful conjugation of 83-14 mAb using a heterobifunctional linker resulted in 5-6 molecules of fluorescently labeled 83-14 mAb per SNP.
Functionalized SNPs were taken up by hCMEC/D3 cells efficiently without showing a significant toxic effect on cells of the blood-brain barrier and HepG2 cells.
These results indicate that functionalized PEG-b-PCL SNPs are a promising candidate to deliver drugs to the CNS.
American Psychological Association (APA)
Grossen, Philip& Québatte, Gabriela& Witzigmann, Dominik& Prescianotto-Baschong, Cristina& Dieu, Le-Ha& Huwyler, Jörg. 2016. Functionalized Solid-Sphere PEG-b-PCL Nanoparticles to Target Brain Capillary Endothelial Cells In Vitro. Journal of Nanomaterials،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1109382
Modern Language Association (MLA)
Grossen, Philip…[et al.]. Functionalized Solid-Sphere PEG-b-PCL Nanoparticles to Target Brain Capillary Endothelial Cells In Vitro. Journal of Nanomaterials No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1109382
American Medical Association (AMA)
Grossen, Philip& Québatte, Gabriela& Witzigmann, Dominik& Prescianotto-Baschong, Cristina& Dieu, Le-Ha& Huwyler, Jörg. Functionalized Solid-Sphere PEG-b-PCL Nanoparticles to Target Brain Capillary Endothelial Cells In Vitro. Journal of Nanomaterials. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1109382
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1109382