Preservation of Preloaded DMEK Lenticules in Dextran and Non-Dextran-Based Organ Culture Medium
المؤلفون المشاركون
Parekh, Mohit
Ruzza, Alessandro
Ferrari, Stefano
Ponzin, Diego
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-11-22
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Purpose.
To determine the optimum preservation conditions for preloading DMEK lenticules using organ culture system.
Methods.
8.5 mm DMEK lenticules were stripped and preserved with endothelium flap-in for 4 days at RT in an IOL cartridge that was blocked with rubber stoppers from each end.
In C1, tissues were collected from tissue culture medium (TCM) and preserved in TCM.
In C2, tissues were collected from transport medium (TCM + 6% dextran T500) (TM) and preserved in TM.
In C3, tissues were collected from TCM and preserved in TM.
Mortality, glucose uptake, histological staining, tight junctions and cell apoptosis were studied post-preservation.
Results.
Mortality in C1, C2, and C3 were 49.40%, 8.53%, and 27.74%, with 40.7%, 13%, and 41.8% uncovered areas.
Glucose uptake (mg/mL) was 0.32, 0.43, and 0.56 in C1, C2, and C3.
PAS staining showed presence of DM and endothelium in C2 but not in C1 and with fewer cells in C3.
ZO-1 was expressed in all the conditions.
Polymorphism was higher in C1 and C3.
Mild apoptosis was observed in C3.
Conclusions.
Dextran may play an important role in preserving the endothelial cells before and after stripping for trifolded (endothelium-in) preloaded DMEK lenticules.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Parekh, Mohit& Ruzza, Alessandro& Ferrari, Stefano& Ponzin, Diego. 2016. Preservation of Preloaded DMEK Lenticules in Dextran and Non-Dextran-Based Organ Culture Medium. Journal of Ophthalmology،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1110009
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Parekh, Mohit…[et al.]. Preservation of Preloaded DMEK Lenticules in Dextran and Non-Dextran-Based Organ Culture Medium. Journal of Ophthalmology No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1110009
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Parekh, Mohit& Ruzza, Alessandro& Ferrari, Stefano& Ponzin, Diego. Preservation of Preloaded DMEK Lenticules in Dextran and Non-Dextran-Based Organ Culture Medium. Journal of Ophthalmology. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1110009
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1110009
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر