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Preservation of Preloaded DMEK Lenticules in Dextran and Non-Dextran-Based Organ Culture Medium
Joint Authors
Parekh, Mohit
Ruzza, Alessandro
Ferrari, Stefano
Ponzin, Diego
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-22
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Purpose.
To determine the optimum preservation conditions for preloading DMEK lenticules using organ culture system.
Methods.
8.5 mm DMEK lenticules were stripped and preserved with endothelium flap-in for 4 days at RT in an IOL cartridge that was blocked with rubber stoppers from each end.
In C1, tissues were collected from tissue culture medium (TCM) and preserved in TCM.
In C2, tissues were collected from transport medium (TCM + 6% dextran T500) (TM) and preserved in TM.
In C3, tissues were collected from TCM and preserved in TM.
Mortality, glucose uptake, histological staining, tight junctions and cell apoptosis were studied post-preservation.
Results.
Mortality in C1, C2, and C3 were 49.40%, 8.53%, and 27.74%, with 40.7%, 13%, and 41.8% uncovered areas.
Glucose uptake (mg/mL) was 0.32, 0.43, and 0.56 in C1, C2, and C3.
PAS staining showed presence of DM and endothelium in C2 but not in C1 and with fewer cells in C3.
ZO-1 was expressed in all the conditions.
Polymorphism was higher in C1 and C3.
Mild apoptosis was observed in C3.
Conclusions.
Dextran may play an important role in preserving the endothelial cells before and after stripping for trifolded (endothelium-in) preloaded DMEK lenticules.
American Psychological Association (APA)
Parekh, Mohit& Ruzza, Alessandro& Ferrari, Stefano& Ponzin, Diego. 2016. Preservation of Preloaded DMEK Lenticules in Dextran and Non-Dextran-Based Organ Culture Medium. Journal of Ophthalmology،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1110009
Modern Language Association (MLA)
Parekh, Mohit…[et al.]. Preservation of Preloaded DMEK Lenticules in Dextran and Non-Dextran-Based Organ Culture Medium. Journal of Ophthalmology No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1110009
American Medical Association (AMA)
Parekh, Mohit& Ruzza, Alessandro& Ferrari, Stefano& Ponzin, Diego. Preservation of Preloaded DMEK Lenticules in Dextran and Non-Dextran-Based Organ Culture Medium. Journal of Ophthalmology. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1110009
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1110009