An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits

المؤلفون المشاركون

Xie, Shang
Xu, Hui
Lin, Bo
Wang, Kan
Shan, Xiao-Feng
Cai, Zhi-Gang

المصدر

Journal of Ophthalmology

العدد

المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-8، 8ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2016-10-20

دولة النشر

مصر

عدد الصفحات

8

التخصصات الرئيسية

الطب البشري

الملخص EN

Objectives.

To investigate whether botulinum toxin type A (BTXA) could control excessive secretion after submandibular gland (SMG) transplantation in rabbits and its possible mechanisms.

Methods.

A new SMG transplantation model was established in rabbit.

30 successfully constructed models were randomly assigned to five groups including control group and four experimental groups.

Secretion outputs were used to analyze the effect of BTXA injection on excessive secretion.

Hematoxylin and eosin (HE) staining, transmission electron microscopy (TEM), Western blot, and immunofluorescence were performed to analyze its possible mechanisms.

Results.

After BTXA injection, a significant decrease of excessive secretion after SMG transplantation was found in 2 and 4 weeks groups, but no significant effect on 12 and 24 weeks groups.

HE and TEM results showed that BTXA led to morphological and ultrastructural changes of acinar cells of transplanted SMG.

Western blot results suggested that BTXA decreased the aquaporin-5 (AQP5) protein expression after BTXA injection for 2 and 4 weeks.

Immunofluorescence results showed that AQP5 protein was mainly expressed in the cytoplasm after BTXA injection for 2 and 4 weeks, which might indicate that BTXA promoted AQP5 expression from the cell membrane to cytoplasm.

Conclusion.

BTXA could effectively control excessive secretion after SMG transplantation in rabbits.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Xie, Shang& Xu, Hui& Lin, Bo& Wang, Kan& Shan, Xiao-Feng& Cai, Zhi-Gang. 2016. An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits. Journal of Ophthalmology،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1110047

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Xie, Shang…[et al.]. An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits. Journal of Ophthalmology No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1110047

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Xie, Shang& Xu, Hui& Lin, Bo& Wang, Kan& Shan, Xiao-Feng& Cai, Zhi-Gang. An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits. Journal of Ophthalmology. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1110047

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1110047