An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits
Joint Authors
Xie, Shang
Xu, Hui
Lin, Bo
Wang, Kan
Shan, Xiao-Feng
Cai, Zhi-Gang
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objectives.
To investigate whether botulinum toxin type A (BTXA) could control excessive secretion after submandibular gland (SMG) transplantation in rabbits and its possible mechanisms.
Methods.
A new SMG transplantation model was established in rabbit.
30 successfully constructed models were randomly assigned to five groups including control group and four experimental groups.
Secretion outputs were used to analyze the effect of BTXA injection on excessive secretion.
Hematoxylin and eosin (HE) staining, transmission electron microscopy (TEM), Western blot, and immunofluorescence were performed to analyze its possible mechanisms.
Results.
After BTXA injection, a significant decrease of excessive secretion after SMG transplantation was found in 2 and 4 weeks groups, but no significant effect on 12 and 24 weeks groups.
HE and TEM results showed that BTXA led to morphological and ultrastructural changes of acinar cells of transplanted SMG.
Western blot results suggested that BTXA decreased the aquaporin-5 (AQP5) protein expression after BTXA injection for 2 and 4 weeks.
Immunofluorescence results showed that AQP5 protein was mainly expressed in the cytoplasm after BTXA injection for 2 and 4 weeks, which might indicate that BTXA promoted AQP5 expression from the cell membrane to cytoplasm.
Conclusion.
BTXA could effectively control excessive secretion after SMG transplantation in rabbits.
American Psychological Association (APA)
Xie, Shang& Xu, Hui& Lin, Bo& Wang, Kan& Shan, Xiao-Feng& Cai, Zhi-Gang. 2016. An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits. Journal of Ophthalmology،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1110047
Modern Language Association (MLA)
Xie, Shang…[et al.]. An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits. Journal of Ophthalmology No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1110047
American Medical Association (AMA)
Xie, Shang& Xu, Hui& Lin, Bo& Wang, Kan& Shan, Xiao-Feng& Cai, Zhi-Gang. An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits. Journal of Ophthalmology. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1110047
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1110047