Effect of Interferon-γ on the Basal and the TNFα-Stimulated Secretion of CXCL8 in Thyroid Cancer Cell Lines Bearing Either the RETPTC Rearrangement Or the BRAF V600e Mutation
المؤلفون المشاركون
Rotondi, Mario
Coperchini, Francesca
Awwad, Oriana
Pignatti, Patrizia
Di Buduo, Christian A.
Abbonante, Vittorio
Magri, Flavia
Balduini, Alessandra
Chiovato, Luca
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-07-31
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
CXCL8 displays several tumor-promoting effects.
Targeting and/or lowering CXCL8 concentrations within the tumor microenvironment would produce a therapeutic benefit.
Aim of this study was to test the effect of IFNγ on the basal and TNFα-stimulated secretion of CXCL8 in TCP-1 and BCPAP thyroid cancer cell lines (harboring RET/PTC rearrangement and BRAF V600e mutation, resp.).
Cells were incubated with IFNγ (1, 10, 100, and 1000 U/mL) alone or in combination with TNF-α (10 ng/mL) for 24 hours.
CXCL8 and CXCL10 concentrations were measured in the cell supernatants.
IFNγ inhibited in a dose-dependent and significant manner both the basal (ANOVA F: 22.759; p < 0.00001 ) and the TNFα-stimulated (ANOVA F: 15.309; p < 0.00001 ) CXCL8 secretions in BCPAP but not in TPC-1 cells (NS).
On the other hand, IFNγ and IFNγ + TNF-α induced a significant secretion of CXCL10 in both BCPAP ( p < 0.05 ) and TPC-1 ( p < 0.05 ) cells.
Transwell migration assay showed that (i) CXCL8 increased cell migration in both TPC-1 and BCPAP cells; (ii) IFNγ significantly reduced the migration only of BCPAP cells; and (iii) CXCL8 reverted the effect of IFNγ.
These results constitute the first demonstration that IFNγ inhibits CXCL8 secretion and in turn the migration of a BRAF V600e mutated thyroid cell line.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Rotondi, Mario& Coperchini, Francesca& Awwad, Oriana& Pignatti, Patrizia& Di Buduo, Christian A.& Abbonante, Vittorio…[et al.]. 2016. Effect of Interferon-γ on the Basal and the TNFα-Stimulated Secretion of CXCL8 in Thyroid Cancer Cell Lines Bearing Either the RETPTC Rearrangement Or the BRAF V600e Mutation. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1111262
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Rotondi, Mario…[et al.]. Effect of Interferon-γ on the Basal and the TNFα-Stimulated Secretion of CXCL8 in Thyroid Cancer Cell Lines Bearing Either the RETPTC Rearrangement Or the BRAF V600e Mutation. Mediators of Inflammation No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1111262
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Rotondi, Mario& Coperchini, Francesca& Awwad, Oriana& Pignatti, Patrizia& Di Buduo, Christian A.& Abbonante, Vittorio…[et al.]. Effect of Interferon-γ on the Basal and the TNFα-Stimulated Secretion of CXCL8 in Thyroid Cancer Cell Lines Bearing Either the RETPTC Rearrangement Or the BRAF V600e Mutation. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1111262
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1111262
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر