Effect of Interferon-γ on the Basal and the TNFα-Stimulated Secretion of CXCL8 in Thyroid Cancer Cell Lines Bearing Either the RETPTC Rearrangement Or the BRAF V600e Mutation
Joint Authors
Rotondi, Mario
Coperchini, Francesca
Awwad, Oriana
Pignatti, Patrizia
Di Buduo, Christian A.
Abbonante, Vittorio
Magri, Flavia
Balduini, Alessandra
Chiovato, Luca
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-31
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
CXCL8 displays several tumor-promoting effects.
Targeting and/or lowering CXCL8 concentrations within the tumor microenvironment would produce a therapeutic benefit.
Aim of this study was to test the effect of IFNγ on the basal and TNFα-stimulated secretion of CXCL8 in TCP-1 and BCPAP thyroid cancer cell lines (harboring RET/PTC rearrangement and BRAF V600e mutation, resp.).
Cells were incubated with IFNγ (1, 10, 100, and 1000 U/mL) alone or in combination with TNF-α (10 ng/mL) for 24 hours.
CXCL8 and CXCL10 concentrations were measured in the cell supernatants.
IFNγ inhibited in a dose-dependent and significant manner both the basal (ANOVA F: 22.759; p < 0.00001 ) and the TNFα-stimulated (ANOVA F: 15.309; p < 0.00001 ) CXCL8 secretions in BCPAP but not in TPC-1 cells (NS).
On the other hand, IFNγ and IFNγ + TNF-α induced a significant secretion of CXCL10 in both BCPAP ( p < 0.05 ) and TPC-1 ( p < 0.05 ) cells.
Transwell migration assay showed that (i) CXCL8 increased cell migration in both TPC-1 and BCPAP cells; (ii) IFNγ significantly reduced the migration only of BCPAP cells; and (iii) CXCL8 reverted the effect of IFNγ.
These results constitute the first demonstration that IFNγ inhibits CXCL8 secretion and in turn the migration of a BRAF V600e mutated thyroid cell line.
American Psychological Association (APA)
Rotondi, Mario& Coperchini, Francesca& Awwad, Oriana& Pignatti, Patrizia& Di Buduo, Christian A.& Abbonante, Vittorio…[et al.]. 2016. Effect of Interferon-γ on the Basal and the TNFα-Stimulated Secretion of CXCL8 in Thyroid Cancer Cell Lines Bearing Either the RETPTC Rearrangement Or the BRAF V600e Mutation. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1111262
Modern Language Association (MLA)
Rotondi, Mario…[et al.]. Effect of Interferon-γ on the Basal and the TNFα-Stimulated Secretion of CXCL8 in Thyroid Cancer Cell Lines Bearing Either the RETPTC Rearrangement Or the BRAF V600e Mutation. Mediators of Inflammation No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1111262
American Medical Association (AMA)
Rotondi, Mario& Coperchini, Francesca& Awwad, Oriana& Pignatti, Patrizia& Di Buduo, Christian A.& Abbonante, Vittorio…[et al.]. Effect of Interferon-γ on the Basal and the TNFα-Stimulated Secretion of CXCL8 in Thyroid Cancer Cell Lines Bearing Either the RETPTC Rearrangement Or the BRAF V600e Mutation. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1111262
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1111262