SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells
المؤلفون المشاركون
Jia, Ji
Zhang, Lei
Shi, Xiaolei
Wu, Mingchun
Zhou, Xiang
Liu, Xiaonan
Huo, Tingting
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-12-07
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Background.
Cytoprotectant amifostine attenuates radiation-induced oxidative injury by increasing intracellular manganese superoxide dismutase (SOD2) in peripheral tissue.
However, whether amifostine could protect neuronal cells against oxidative injury has not been reported.
The purpose of this study is to explore the protection of amifostine in PC12 cells.
Methods.
PC12 cells exposed to glutamate were used to mimic neuronal oxidative injury.
SOD assay kit was taken to evaluate intracellular Cu/Zn SOD (SOD1) and SOD2 activities; western blot analysis and immunofluorescence staining were performed to investigate SOD2 protein expression; MTT, lactate dehydrogenase (LDH), release and cell morphology were used to evaluate cell injury degree, and apoptotic rate and cleaved caspase-3 expression were taken to assess apoptosis; mitochondrial superoxide production, intracellular reactive oxygen species (ROS), and glutathione (GSH) and catalase (CAT) levels were evaluated by reagent kits.
Results.
Amifostine increased SOD2 activity and expression, decreased cell injury and apoptosis, reduced mitochondrial superoxide production and intracellular ROS generation, and restored intracellular GSH and CAT levels in PC12 cells exposed to glutamate.
SOD2-siRNA, however, significantly reversed the amifostine-induced cytoprotective and antioxidative actions.
Conclusion.
SOD2 mediates amifostine-induced protection in PC12 cells exposed to glutamate.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jia, Ji& Zhang, Lei& Shi, Xiaolei& Wu, Mingchun& Zhou, Xiang& Liu, Xiaonan…[et al.]. 2015. SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1113909
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jia, Ji…[et al.]. SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1113909
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jia, Ji& Zhang, Lei& Shi, Xiaolei& Wu, Mingchun& Zhou, Xiang& Liu, Xiaonan…[et al.]. SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1113909
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1113909
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر