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SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells
Joint Authors
Jia, Ji
Zhang, Lei
Shi, Xiaolei
Wu, Mingchun
Zhou, Xiang
Liu, Xiaonan
Huo, Tingting
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Cytoprotectant amifostine attenuates radiation-induced oxidative injury by increasing intracellular manganese superoxide dismutase (SOD2) in peripheral tissue.
However, whether amifostine could protect neuronal cells against oxidative injury has not been reported.
The purpose of this study is to explore the protection of amifostine in PC12 cells.
Methods.
PC12 cells exposed to glutamate were used to mimic neuronal oxidative injury.
SOD assay kit was taken to evaluate intracellular Cu/Zn SOD (SOD1) and SOD2 activities; western blot analysis and immunofluorescence staining were performed to investigate SOD2 protein expression; MTT, lactate dehydrogenase (LDH), release and cell morphology were used to evaluate cell injury degree, and apoptotic rate and cleaved caspase-3 expression were taken to assess apoptosis; mitochondrial superoxide production, intracellular reactive oxygen species (ROS), and glutathione (GSH) and catalase (CAT) levels were evaluated by reagent kits.
Results.
Amifostine increased SOD2 activity and expression, decreased cell injury and apoptosis, reduced mitochondrial superoxide production and intracellular ROS generation, and restored intracellular GSH and CAT levels in PC12 cells exposed to glutamate.
SOD2-siRNA, however, significantly reversed the amifostine-induced cytoprotective and antioxidative actions.
Conclusion.
SOD2 mediates amifostine-induced protection in PC12 cells exposed to glutamate.
American Psychological Association (APA)
Jia, Ji& Zhang, Lei& Shi, Xiaolei& Wu, Mingchun& Zhou, Xiang& Liu, Xiaonan…[et al.]. 2015. SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1113909
Modern Language Association (MLA)
Jia, Ji…[et al.]. SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1113909
American Medical Association (AMA)
Jia, Ji& Zhang, Lei& Shi, Xiaolei& Wu, Mingchun& Zhou, Xiang& Liu, Xiaonan…[et al.]. SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1113909
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113909