Equine Metabolic Syndrome Affects Viability, Senescence, and Stress Factors of Equine Adipose-Derived Mesenchymal Stromal Stem Cells: New Insight into EqASCs Isolated from EMS Horses in the Context of Their Aging
المؤلفون المشاركون
Czyrek, Aleksandra
Kornicka, Katarzyna
Basinska, Katarzyna
Marycz, Krzysztof
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-11-22
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
Currently, equine metabolic syndrome (EMS), an endocrine disease linked to insulin resistance, affects an increasing number of horses.
However, little is known about the effect of EMS on mesenchymal stem cells that reside in adipose tissue (ASC).
Thus it is crucial to evaluate the viability and growth kinetics of these cells, particularly in terms of their application in regenerative medicine.
In this study, we investigated the proliferative capacity, morphological features, and accumulation of oxidative stress factors in mesenchymal stem cells isolated from healthy animals (ASCN) and horses suffering from EMS (ASCEMS).
ASCEMS displayed senescent phenotype associated with β-galactosidase accumulation, enlarged cell bodies and nuclei, increased apoptosis, and reduced heterochromatin architecture.
Moreover, we observed increased amounts of nitric oxide (NO) and reactive oxygen species (ROS) in these cells, accompanied by reduced superoxide dismutase (SOD) activity.
We also found in ASCEMS an elevated number of impaired mitochondria, characterized by membrane raptures, disarrayed cristae, and vacuole formation.
Our results suggest that the toxic compounds, accumulating in the mitochondria under oxidative stress, lead to alternations in their morphology and may be partially responsible for the senescent phenotype and decreased proliferation potential of ASCEMS.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Marycz, Krzysztof& Kornicka, Katarzyna& Basinska, Katarzyna& Czyrek, Aleksandra. 2015. Equine Metabolic Syndrome Affects Viability, Senescence, and Stress Factors of Equine Adipose-Derived Mesenchymal Stromal Stem Cells: New Insight into EqASCs Isolated from EMS Horses in the Context of Their Aging. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1113948
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Marycz, Krzysztof…[et al.]. Equine Metabolic Syndrome Affects Viability, Senescence, and Stress Factors of Equine Adipose-Derived Mesenchymal Stromal Stem Cells: New Insight into EqASCs Isolated from EMS Horses in the Context of Their Aging. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-17.
https://search.emarefa.net/detail/BIM-1113948
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Marycz, Krzysztof& Kornicka, Katarzyna& Basinska, Katarzyna& Czyrek, Aleksandra. Equine Metabolic Syndrome Affects Viability, Senescence, and Stress Factors of Equine Adipose-Derived Mesenchymal Stromal Stem Cells: New Insight into EqASCs Isolated from EMS Horses in the Context of Their Aging. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1113948
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1113948
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر