Equine Metabolic Syndrome Affects Viability, Senescence, and Stress Factors of Equine Adipose-Derived Mesenchymal Stromal Stem Cells: New Insight into EqASCs Isolated from EMS Horses in the Context of Their Aging

Joint Authors

Czyrek, Aleksandra
Kornicka, Katarzyna
Basinska, Katarzyna
Marycz, Krzysztof

Source

Oxidative Medicine and Cellular Longevity

Issue

Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-17, 17 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2015-11-22

Country of Publication

Egypt

No. of Pages

17

Main Subjects

Biology

Abstract EN

Currently, equine metabolic syndrome (EMS), an endocrine disease linked to insulin resistance, affects an increasing number of horses.

However, little is known about the effect of EMS on mesenchymal stem cells that reside in adipose tissue (ASC).

Thus it is crucial to evaluate the viability and growth kinetics of these cells, particularly in terms of their application in regenerative medicine.

In this study, we investigated the proliferative capacity, morphological features, and accumulation of oxidative stress factors in mesenchymal stem cells isolated from healthy animals (ASCN) and horses suffering from EMS (ASCEMS).

ASCEMS displayed senescent phenotype associated with β-galactosidase accumulation, enlarged cell bodies and nuclei, increased apoptosis, and reduced heterochromatin architecture.

Moreover, we observed increased amounts of nitric oxide (NO) and reactive oxygen species (ROS) in these cells, accompanied by reduced superoxide dismutase (SOD) activity.

We also found in ASCEMS an elevated number of impaired mitochondria, characterized by membrane raptures, disarrayed cristae, and vacuole formation.

Our results suggest that the toxic compounds, accumulating in the mitochondria under oxidative stress, lead to alternations in their morphology and may be partially responsible for the senescent phenotype and decreased proliferation potential of ASCEMS.

American Psychological Association (APA)

Marycz, Krzysztof& Kornicka, Katarzyna& Basinska, Katarzyna& Czyrek, Aleksandra. 2015. Equine Metabolic Syndrome Affects Viability, Senescence, and Stress Factors of Equine Adipose-Derived Mesenchymal Stromal Stem Cells: New Insight into EqASCs Isolated from EMS Horses in the Context of Their Aging. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1113948

Modern Language Association (MLA)

Marycz, Krzysztof…[et al.]. Equine Metabolic Syndrome Affects Viability, Senescence, and Stress Factors of Equine Adipose-Derived Mesenchymal Stromal Stem Cells: New Insight into EqASCs Isolated from EMS Horses in the Context of Their Aging. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-17.
https://search.emarefa.net/detail/BIM-1113948

American Medical Association (AMA)

Marycz, Krzysztof& Kornicka, Katarzyna& Basinska, Katarzyna& Czyrek, Aleksandra. Equine Metabolic Syndrome Affects Viability, Senescence, and Stress Factors of Equine Adipose-Derived Mesenchymal Stromal Stem Cells: New Insight into EqASCs Isolated from EMS Horses in the Context of Their Aging. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1113948

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1113948