GST-TAT-SOD: Cell Permeable Bifunctional Antioxidant Enzyme—A Potential Selective Radioprotector
المؤلفون المشاركون
Liu, Shu-Tao
Wu, Jun-xin
Pan, Jianru
He, Huocong
Su, Ying
Zheng, Guangjin
Rao, Pingfan
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-05-30
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Superoxide dismutase (SOD) fusion of TAT was proved to be radioprotective in our previous work.
On that basis, a bifunctional recombinant protein which was the fusion of glutathione S-transferase (GST), SOD, and TAT was constructed and named GST-TAT-SOD.
Herein we report the investigation of the cytotoxicity, cell-penetrating activity, and in vitro radioprotective effect of GST-TAT-SOD compared with wild SOD, single-function recombinant protein SOD-TAT, and amifostine.
We demonstrated that wild SOD had little radioprotective effect on irradiated L-02 and Hep G2 cells while amifostine was protective to both cell lines.
SOD-TAT or GST-TAT-SOD pretreatment 3 h prior to radiation protects irradiated normal liver cells against radiation damage by eliminating intracellular excrescent superoxide, reducing cellular MDA level, enhancing cellular antioxidant ability and colony formation ability, and reducing apoptosis rate.
Compared with SOD-TAT, GST-TAT-SOD was proved to have better protective effect on irradiated normal liver cells and minimal effect on irradiated hepatoma cells.
Besides, GST-TAT-SOD was safe for normal cells and effectively transduced into different organs in mice, including the brain.
The characteristics of this protein suggest that it may be a potential radioprotective agent in cancer therapy better than amifostine.
Fusion of two antioxidant enzymes and cell-penetrating peptides is potentially valuable in the development of radioprotective agent.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Pan, Jianru& He, Huocong& Su, Ying& Zheng, Guangjin& Wu, Jun-xin& Liu, Shu-Tao…[et al.]. 2016. GST-TAT-SOD: Cell Permeable Bifunctional Antioxidant Enzyme—A Potential Selective Radioprotector. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1114071
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Pan, Jianru…[et al.]. GST-TAT-SOD: Cell Permeable Bifunctional Antioxidant Enzyme—A Potential Selective Radioprotector. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1114071
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Pan, Jianru& He, Huocong& Su, Ying& Zheng, Guangjin& Wu, Jun-xin& Liu, Shu-Tao…[et al.]. GST-TAT-SOD: Cell Permeable Bifunctional Antioxidant Enzyme—A Potential Selective Radioprotector. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1114071
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1114071
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر