GST-TAT-SOD: Cell Permeable Bifunctional Antioxidant Enzyme—A Potential Selective Radioprotector
Joint Authors
Liu, Shu-Tao
Wu, Jun-xin
Pan, Jianru
He, Huocong
Su, Ying
Zheng, Guangjin
Rao, Pingfan
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-30
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Superoxide dismutase (SOD) fusion of TAT was proved to be radioprotective in our previous work.
On that basis, a bifunctional recombinant protein which was the fusion of glutathione S-transferase (GST), SOD, and TAT was constructed and named GST-TAT-SOD.
Herein we report the investigation of the cytotoxicity, cell-penetrating activity, and in vitro radioprotective effect of GST-TAT-SOD compared with wild SOD, single-function recombinant protein SOD-TAT, and amifostine.
We demonstrated that wild SOD had little radioprotective effect on irradiated L-02 and Hep G2 cells while amifostine was protective to both cell lines.
SOD-TAT or GST-TAT-SOD pretreatment 3 h prior to radiation protects irradiated normal liver cells against radiation damage by eliminating intracellular excrescent superoxide, reducing cellular MDA level, enhancing cellular antioxidant ability and colony formation ability, and reducing apoptosis rate.
Compared with SOD-TAT, GST-TAT-SOD was proved to have better protective effect on irradiated normal liver cells and minimal effect on irradiated hepatoma cells.
Besides, GST-TAT-SOD was safe for normal cells and effectively transduced into different organs in mice, including the brain.
The characteristics of this protein suggest that it may be a potential radioprotective agent in cancer therapy better than amifostine.
Fusion of two antioxidant enzymes and cell-penetrating peptides is potentially valuable in the development of radioprotective agent.
American Psychological Association (APA)
Pan, Jianru& He, Huocong& Su, Ying& Zheng, Guangjin& Wu, Jun-xin& Liu, Shu-Tao…[et al.]. 2016. GST-TAT-SOD: Cell Permeable Bifunctional Antioxidant Enzyme—A Potential Selective Radioprotector. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1114071
Modern Language Association (MLA)
Pan, Jianru…[et al.]. GST-TAT-SOD: Cell Permeable Bifunctional Antioxidant Enzyme—A Potential Selective Radioprotector. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1114071
American Medical Association (AMA)
Pan, Jianru& He, Huocong& Su, Ying& Zheng, Guangjin& Wu, Jun-xin& Liu, Shu-Tao…[et al.]. GST-TAT-SOD: Cell Permeable Bifunctional Antioxidant Enzyme—A Potential Selective Radioprotector. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1114071
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114071