The Natural Polyphenol Epigallocatechin Gallate Protects Intervertebral Disc Cells from Oxidative Stress
المؤلفون المشاركون
Ferguson, Stephen J.
Krupkova, Olga
Handa, Junichi
Hlavna, Marian
Klasen, Juergen
Ospelt, Caroline
Wuertz-Kozak, Karin
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-03-28
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
Oxidative stress-related phenotypic changes and a decline in the number of viable cells are crucial contributors to intervertebral disc degeneration.
The polyphenol epigallocatechin 3-gallate (EGCG) can interfere with painful disc degeneration by reducing inflammation, catabolism, and pain.
In this study, we hypothesized that EGCG furthermore protects against senescence and/or cell death, induced by oxidative stress.
Sublethal and lethal oxidative stress were induced in primary human intervertebral disc cells with H2O2 (total n = 36 ).
Under sublethal conditions, the effects of EGCG on p53-p21 activation, proliferative capacity, and accumulation of senescence-associated β-galactosidase were tested.
Further, the effects of EGCG on mitochondria depolarization and cell viability were analyzed in lethal oxidative stress.
The inhibitor LY249002 was applied to investigate the PI3K/Akt pathway.
EGCG inhibited accumulation of senescence-associated β-galactosidase but did not affect the loss of proliferative capacity, suggesting that EGCG did not fully neutralize exogenous radicals.
Furthermore, EGCG increased the survival of IVD cells in lethal oxidative stress via activation of prosurvival PI3K/Akt and protection of mitochondria.
We demonstrated that EGCG not only inhibits inflammation but also can enhance the survival of disc cells in oxidative stress, which makes it a suitable candidate for the development of novel therapies targeting disc degeneration.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Krupkova, Olga& Handa, Junichi& Hlavna, Marian& Klasen, Juergen& Ospelt, Caroline& Ferguson, Stephen J.…[et al.]. 2016. The Natural Polyphenol Epigallocatechin Gallate Protects Intervertebral Disc Cells from Oxidative Stress. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1114165
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Krupkova, Olga…[et al.]. The Natural Polyphenol Epigallocatechin Gallate Protects Intervertebral Disc Cells from Oxidative Stress. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-17.
https://search.emarefa.net/detail/BIM-1114165
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Krupkova, Olga& Handa, Junichi& Hlavna, Marian& Klasen, Juergen& Ospelt, Caroline& Ferguson, Stephen J.…[et al.]. The Natural Polyphenol Epigallocatechin Gallate Protects Intervertebral Disc Cells from Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1114165
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1114165
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر