The Natural Polyphenol Epigallocatechin Gallate Protects Intervertebral Disc Cells from Oxidative Stress

Joint Authors

Ferguson, Stephen J.
Krupkova, Olga
Handa, Junichi
Hlavna, Marian
Klasen, Juergen
Ospelt, Caroline
Wuertz-Kozak, Karin

Source

Oxidative Medicine and Cellular Longevity

Issue

Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-17, 17 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2016-03-28

Country of Publication

Egypt

No. of Pages

17

Main Subjects

Biology

Abstract EN

Oxidative stress-related phenotypic changes and a decline in the number of viable cells are crucial contributors to intervertebral disc degeneration.

The polyphenol epigallocatechin 3-gallate (EGCG) can interfere with painful disc degeneration by reducing inflammation, catabolism, and pain.

In this study, we hypothesized that EGCG furthermore protects against senescence and/or cell death, induced by oxidative stress.

Sublethal and lethal oxidative stress were induced in primary human intervertebral disc cells with H2O2 (total n = 36 ).

Under sublethal conditions, the effects of EGCG on p53-p21 activation, proliferative capacity, and accumulation of senescence-associated β-galactosidase were tested.

Further, the effects of EGCG on mitochondria depolarization and cell viability were analyzed in lethal oxidative stress.

The inhibitor LY249002 was applied to investigate the PI3K/Akt pathway.

EGCG inhibited accumulation of senescence-associated β-galactosidase but did not affect the loss of proliferative capacity, suggesting that EGCG did not fully neutralize exogenous radicals.

Furthermore, EGCG increased the survival of IVD cells in lethal oxidative stress via activation of prosurvival PI3K/Akt and protection of mitochondria.

We demonstrated that EGCG not only inhibits inflammation but also can enhance the survival of disc cells in oxidative stress, which makes it a suitable candidate for the development of novel therapies targeting disc degeneration.

American Psychological Association (APA)

Krupkova, Olga& Handa, Junichi& Hlavna, Marian& Klasen, Juergen& Ospelt, Caroline& Ferguson, Stephen J.…[et al.]. 2016. The Natural Polyphenol Epigallocatechin Gallate Protects Intervertebral Disc Cells from Oxidative Stress. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1114165

Modern Language Association (MLA)

Krupkova, Olga…[et al.]. The Natural Polyphenol Epigallocatechin Gallate Protects Intervertebral Disc Cells from Oxidative Stress. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-17.
https://search.emarefa.net/detail/BIM-1114165

American Medical Association (AMA)

Krupkova, Olga& Handa, Junichi& Hlavna, Marian& Klasen, Juergen& Ospelt, Caroline& Ferguson, Stephen J.…[et al.]. The Natural Polyphenol Epigallocatechin Gallate Protects Intervertebral Disc Cells from Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1114165

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1114165