Effects of Epigallocatechin Gallate on Tert-Butyl Hydroperoxide-Induced Mitochondrial Dysfunction in Rat Liver Mitochondria and Hepatocytes
المؤلفون المشاركون
Kučera, Otto
Endlicher, René
Červinková, Zuzana
Mezera, Vojtech
Sobotka, Ondrej
Drahota, Zdenek
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-12-15
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Epigallocatechin gallate (EGCG) is a green tea antioxidant with adverse effects on rat liver mitochondria and hepatocytes at high doses.
Here, we assessed whether low doses of EGCG would protect these systems from damage induced by tert-butyl hydroperoxide (tBHP).
Rat liver mitochondria or permeabilized rat hepatocytes were pretreated with EGCG and then exposed to tBHP.
Oxygen consumption, mitochondrial membrane potential (MMP), and mitochondrial retention capacity for calcium were measured.
First, 50 μM EGCG or 0.25 mM tBHP alone increased State 4 Complex I-driven respiration, thus demonstrating uncoupling effects; tBHP also inhibited State 3 ADP-stimulated respiration.
Then, the coexposure to 0.25 mM tBHP and 50 μM EGCG induced a trend of further decline in the respiratory control ratio beyond that observed upon tBHP exposure alone.
EGCG had no effect on MMP and no effect, in concentrations up to 50 μM, on mitochondrial calcium retention capacity.
tBHP led to a decline in both MMP and mitochondrial retention capacity for calcium; these effects were not changed by pretreatment with EGCG.
In addition, EGCG dose-dependently enhanced hydrogen peroxide formation in a cell- and mitochondria-free medium.
Conclusion.
Moderate nontoxic doses of EGCG were not able to protect rat liver mitochondria and hepatocytes from tBHP-induced mitochondrial dysfunction.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mezera, Vojtech& Endlicher, René& Kučera, Otto& Sobotka, Ondrej& Drahota, Zdenek& Červinková, Zuzana. 2016. Effects of Epigallocatechin Gallate on Tert-Butyl Hydroperoxide-Induced Mitochondrial Dysfunction in Rat Liver Mitochondria and Hepatocytes. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1114215
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mezera, Vojtech…[et al.]. Effects of Epigallocatechin Gallate on Tert-Butyl Hydroperoxide-Induced Mitochondrial Dysfunction in Rat Liver Mitochondria and Hepatocytes. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1114215
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mezera, Vojtech& Endlicher, René& Kučera, Otto& Sobotka, Ondrej& Drahota, Zdenek& Červinková, Zuzana. Effects of Epigallocatechin Gallate on Tert-Butyl Hydroperoxide-Induced Mitochondrial Dysfunction in Rat Liver Mitochondria and Hepatocytes. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1114215
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1114215
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر